Metal sulfide quantum dots-aggregated PAMAM dendrimer for cadmium ion-selective electrode-based immunoassay of alpha-fetoprotein

被引：0

作者：

Qunfang Li ^{[1
,2
]}

Jing Jin ^{[1
]}

Fangming Lou ^{[1
]}

Dianping Tang ^{[2
]}

机构：

[1] School of Chemistry and Environmental Engineering, Hubei University for Nationalities

[2] Key Laboratory for Analytical Science of Food Safety and Biology of Ministry of Education, Fujian Province, State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University

来源：

Science China(Chemistry) | 2018年 / 61卷 / 06期

关键词：

D O I：

暂无

中图分类号：

O657.1 [电化学分析法];

学科分类号：

070302 ; 081704 ;

摘要：

An ion-selective electrode（ISE）-based immunoassay has been innovatively designed for the sensitive detection of liver cancer biomarker（alpha-fetoprotein,AFP）,using metal sulfide quantum dot（QD）-based nano labels.Cd S QDs-aggregated PAMAM dendrimer（QD-DE）was first synthesized and functionalized with polyclonal rabbit anti-human AFP antibodies.Thereafter,a sandwich immunoreaction was implemented on monoclonal mouse anti-human AFP antibody-coated microplate by using antibody-functionalized QD-DE as the secondary antibody.Accompanying the immunocomplex,subsequent potentiometric detection of cadmium ion dissolved from the QD-DE under acidic condition was conducted on a portable cadmium ion-selective electrode（Cd-ISE）.Results revealed that the electrode potential of the Cd-ISE increased with the increment of AFP concentration from 0.1 to 100 ng m L-1at a detection limit（LOD）of 68 pg m L-1.The relative standard deviations（RSD）were below9.09%and 10.54%for the intra-and inter-assay,respectively.Additionally,six human serum specimens were determined on CdISE-based immunosensor by using commercial human AFP ELISA kit as the reference,and gave good relationship between two methods.Importantly,Cd-ISE-based immunoassay offers the promise for simple and cost-effective screening of disease-related biomarkers.

引用

页码：750 / 756

页数：7