Prevalence of Mycobacterium leprae and Mycobacterium lepromatosis in roadkill armadillos in Brazil

被引：4

作者：

Monsalve-Lara, J. ^{[1
]}

Drummond, M. ^{[2
]}

Romero-Alvarez, D. ^{[3
]}

Velho, P. E. N. F. ^{[2
]}

Jimenez-Garcia, D. ^{[4
]}

Marques, R. ^{[4
]}

Peterson, A. T. ^{[5
,6
]}

Angerami, R. N. ^{[7
]}

Silva, D. P. ^{[2
]}

Donalisio, M. R. ^{[1
]}

机构：

[1] Univ Estadual Campinas UNICAMP, Sch Med Sci, Publ Hlth Dept, Campinas, SP, Brazil

[2] Univ Estadual Campinas UNICAMP, Res Lab Appl Dermatol & Bartonella Infect, Campinas, SP, Brazil

[3] Univ Int SEK UISEK, Hlth Sci Fac, Res Grp Emerging & Neglected Dis Ecoepidemiol & B, Quito, Ecuador

[4] Univ Autonoma Puebla, Ctr Agroecol & Ambiente, Lab Biodivers, Inst Ciencias Benemerita, Puebla, Mexico

[5] Univ Kansas, Biodivers Inst, Lawrence, KS USA

[6] Univ Kansas, Dept Ecol & Evolutionary Biol, Lawrence, KS USA

[7] Univ Estadual Campinas UNICAMP, Sect Hosp Epidemiol, Hosp Clin, Campinas, SP, Brazil

来源：

ACTA TROPICA | 2024年 / 258卷

基金：

巴西圣保罗研究基金会;

关键词：

Mycobacterium leprae; Mycobacterium lepromatosis; Dasypodidae; Leprosy; Zoonosis; 9-BANDED ARMADILLO; ZOONOTIC LEPROSY; INFECTION; DNA; PCR;

D O I：

10.1016/j.actatropica.2024.107333

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

To evaluate the prevalence of Mycobacterium leprae and Mycobacterium lepromatosis in road killed armadillos identified along Brazilian regions, samples of liver, spleen, muscle, ear, nose and tail were collected on highways from 78 animals. The armadillos were of four different species, Cabassous tatouay, Dasypus novemcinctus, Dasypus septemcinctus and Euphractus sexcinctus. After DNA extraction from two tissues, specific primers were used for the detection of each pathogen using SYBR green qualitative Real-Time PCR, and amplicons were sequenced. The species with the highest prevalence was D. novemcinctus, mainly in the Central-West, South, and Southeast regions of Brazil. We detected M. leprae DNA in 32 (41 %) of the 78 individuals and M. lepromatosis DNA was not identified in any of the examined samples. The zoonotic component of leprosy may play a role in the transmission of the disease in endemic areas in which environmental conditions and contact with reservoirs must be investigated.

引用

页数：5

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