Selective recovery of esterase from Trichoderma harzianum through adsorption: Insights on enzymatic catalysis, adsorption isotherms and kinetics

被引:2
作者
Sounderarajan, Sathieesh [1 ,2 ]
Seenivasan, Harshitha [3 ]
Chellam, Padmanaban Velayudhaperumal [4 ]
Puchalapalli, Dinesh Sankar Reddy [1 ]
Ayothiraman, Seenivasan [5 ]
机构
[1] Natl Inst Technol Andhra Pradesh, Dept Chem Engn, Tadepalliguem, Andhra Pradesh, India
[2] Natl Inst Technol Andhra Pradesh, Dept Biotechnol, Biochem Engn Res Grp, Tadepalligudem, Andhra Pradesh, India
[3] Vellore Inst Technol, Sch Biosci & Technol, Vellore, India
[4] Natl Inst Technol Agartala, Dept Bioengn, WATER Res Grp, Agartala, Tripura, India
[5] Govt India, Dept Biotechnol, Natl Inst Technol Andhra Pradesh, Inst Natl Importance, Tadepalligudem 534101, Andhra Pradesh, India
关键词
Esterase; Adsorption; Trichoderma harzianum; Adsorption Isotherm; Thermodynamic analysis; Desorption; Stability analysis; MEMBRANE-IMMOBILIZED ENZYMES; ACTIVATED CARBONS; REMOVAL; BIOTRANSFORMATION; NANOPARTICLES; PURIFICATION; EQUILIBRIUM; ENTRAPMENT; HYDROLYSIS; SORPTION;
D O I
10.1016/j.ijbiomac.2024.134133
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In recent years, numerous attempts have been made to develop a low-cost adsorbent for selectively recovering industrially important products from fermentation broth or complex mixtures. The current study is a novel attempt to selectively adsorb esterase from Trichoderma harzianum using cheap adsorbents like bentonite (BT), activated charcoal (AC), silicon dioxide (SiO2), and titanium dioxide (TiO2). AC had the highest esterase adsorption of 97.58% due to its larger surface area of 594.45 m3/g. SiO2 was found to have the highest selectivity over esterase, with an estimated purification fold of 7.2. Interestingly, the purification fold of 5.5 was found in the BT-extracted fermentation broth. The functional (FT-IR) and morphological analysis (SEM-EDX) were used to characterize the adsorption of esterase. Esterase adsorption on AC, SiO2, and TiO2 was well fitted by Freundlich isotherm, demonstrating multilayer adsorption of esterase. A pseudo-second-order kinetic model was developed for esterase adsorption in various adsorbents. Thermodynamic analysis revealed that adsorption is an endothermic process. AC has the lowest Gibbs free energy of -10.96 kJ/mol, which supports the spontaneous maximum adsorption of both esterase and protein. In the desorption study, the maximum recovery of esterase from TiO2 using sodium chloride was 41.34%. Unlike other adsorbents, the AC-adsorbed esterase maintained its catalytic activity and stability, implying that it could be used as an immobilization system for commercial applications. According to the kinetic analysis, the overall rate of the reaction was controlled by reaction kinetics rather than external mass transfer resistance, as indicated by the Damkohler number.
引用
收藏
页数:16
相关论文
共 98 条
[51]   Preliminary enrichment and separation of genistein and apigenin from extracts of pigeon pea roots by macroporous resins [J].
Liu, Wei ;
Zhang, Su ;
Zu, Yuan-Gang ;
Fu, Yu-Jie ;
Ma, Wei ;
Zhang, Dong-Yang ;
Kong, Yu ;
Li, Xiao-Juan .
BIORESOURCE TECHNOLOGY, 2010, 101 (12) :4667-4675
[52]   Some consideration on the Langmuir isotherm equation [J].
Liu, Y .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2006, 274 (1-3) :34-36
[53]   Optimization of consolidated bioprocessing by response surface methodology in the conversion of corn stover to bioethanol by thermophilic Geobacillus thermoglucosidasius [J].
Madhuvanthi, Sigamani ;
Jayanthi, Singaram ;
Suresh, Subramaniyam ;
Pugazhendhi, Arivalagan .
CHEMOSPHERE, 2022, 304
[54]   Piecewise linear regression: A statistical method for the analysis of experimental adsorption data by the intraparticle-diffusion models [J].
Malash, Gihan F. ;
El-Khaiary, Mohammad I. .
CHEMICAL ENGINEERING JOURNAL, 2010, 163 (03) :256-263
[55]   Activity of an Enzyme Immobilized on Polyelectrolyte Multilayers [J].
Malinin, A. S. ;
Rakhnyanskaya, A. A. ;
Bacheva, A. V. ;
Yaroslavov, A. A. .
POLYMER SCIENCE SERIES A, 2011, 53 (01) :52-56
[56]   Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate [J].
Meneses, Dayana P. ;
Paixao, Livia Maria N. ;
Fonteles, Thatyane, V ;
Gudina, Eduardo J. ;
Rodrigues, Ligia R. ;
Fernandes, Fabiano A. N. ;
Rodrigues, Sueli .
BIOCHEMICAL ENGINEERING JOURNAL, 2021, 168
[57]   Kinetics and thermodynamic study of phosphate adsorption on iron hydroxide-eggshell waste [J].
Mezenner, N. Yeddou ;
Bensmaili, A. .
CHEMICAL ENGINEERING JOURNAL, 2009, 147 (2-3) :87-96
[58]  
Mohammad-Khah A., 2009, International Journal of ChemTech Research, P859, DOI DOI 10.1016/J.FOODCHEM.2017.10.107
[59]   Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel [J].
Mohammadi, Najmeh Sabahi ;
Khiabani, Mahmood Sowti ;
Ghanbarzadeh, Babak ;
Mokarram, Reza Rezaei .
JOURNAL OF BIOTECHNOLOGY, 2020, 323 :189-202
[60]  
Nassef H.M., 2024, Synthesis and characterization of new composite sponge combining of metal-organic framework and chitosan for the elimination of Pb (II), Cu (II) and Cd (II) ions from aqueous solutions: batch adsorption and optimization using Box-Behnken design, V394