Apoptosis-Cell Cycle-Autophagy Molecular Mechanisms Network in Heterogeneous Aggressive Phenotype Prostate Hyperplasia Primary Cell Cultures Have a Prognostic Role

被引：0

作者：

Matei, Elena ^{[1
]}

Enciu, Manuela ^{[2
,3
]}

Rosu, Mihai Catalin ^{[1
]}

Voinea, Felix ^{[3
,4
]}

Mitroi, Anca Florentina ^{[1
,2
]}

Deacu, Mariana ^{[2
,3
]}

Baltatescu, Gabriela Isabela ^{[1
,2
]}

Nicolau, Antonela-Anca ^{[1
,2
]}

Chisoi, Anca ^{[1
]}

Aschie, Mariana ^{[2
,3
]}

Ionescu , Anita Cristina ^{[3
,5
]}

机构：

[1] Ovidius Univ Constanta, Ctr Res & Dev Morphol & Genet Studies Malignant Pa, 145 Tomis Blvd, Constanta 900591, Romania

[2] Sf Apostol Andrei Emergency Cty Hosp, Clin Serv Pathol, 145 Tomis Blvd, Constanta 900591, Romania

[3] Ovidius Univ Constanta, Med Fac, Dept Rheumatol, 1 Univ St, Constanta 900470, Romania

[4] Sf Apostol Andrei Emergency Cty Hosp, Urol Dept, 145 Tomis Blvd, Constanta 900591, Romania

[5] Inst Oncol Prof Dr Alexandru Trestioreanu, Chem Carcinogenesis & Mol Biol Lab, Bucharest 022328, Romania

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 17期

关键词：

caspases; 3/7; activity; cell cycle; aneuploidy status; adhesion glycoproteins; autophagy; nuclear shrinkage; oxidative stress; heterogeneous aggressive phenotype BHP cell cultures; CANCER CELLS; DNA-CONTENT; TUMOR PROGRESSION; OXIDATIVE STRESS; GENE-EXPRESSION; C-MYC; DEATH; CASPASE-3; NECROSIS; INFLAMMATION;

D O I：

10.3390/ijms25179329

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Our study highlights the apoptosis, cell cycle, DNA ploidy, and autophagy molecular mechanisms network to identify prostate pathogenesis and its prognostic role. Caspase 3/7 expressions, cell cycle, adhesion glycoproteins, autophagy, nuclear shrinkage, and oxidative stress by flow-cytometry analysis are used to study the BPH microenvironment's heterogeneity. A high late apoptosis expression by caspases 3/7 activity represents an unfavorable prognostic biomarker, a dependent predictor factor for cell adhesion, growth inhibition by arrest in the G2/M phase, and oxidative stress processes network. The heterogeneous aggressive phenotype prostate adenoma primary cell cultures present a high S-phase category (>12%), with an increased risk of death or recurrence due to aneuploid status presence, representing an unfavorable prognostic biomarker, a dependent predictor factor for caspase 3/7 activity (late apoptosis and necrosis), and cell growth inhibition (G2/M arrest)-linked mechanisms. Increased integrin levels in heterogenous BPH cultures suggest epithelial-mesenchymal transition (EMT) that maintains an aggressive phenotype by escaping cell apoptosis, leading to the cell proliferation necessary in prostate cancer (PCa) development. As predictor biomarkers, the biological mechanisms network involved in apoptosis, the cell cycle, and autophagy help to establish patient prognostic survival or target cancer therapy development.

引用

页数：24