Alginate-based injectable probiotic/squid ink composite hydrogels for accelerated wound healing of MRSA infected abscess through photothermally synergized probiotic therapy

被引：1

作者：

Cui, Yutong ^{[1
]}

Yu, Wenqin ^{[3
]}

Feng, Xiaoshuang ^{[3
]}

Gao, Nannan ^{[2
]}

Chen, Haoyu ^{[3
]}

Wang, Kaiyuan ^{[2
]}

Ren, Wenwen ^{[2
]}

Liu, Yong ^{[2
]}

Li, Juanjuan ^{[3
]}

机构：

[1] Hainan Acad Mangrove, Hainan Acad Forestry, Haikou 571100, Peoples R China

[2] Hainan Univ, Sch Life & Hlth Sci, Haikou 570228, Peoples R China

[3] Hainan Univ, Sch Chem & Chem Engn, Haikou 570228, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 279卷

基金：

中国国家自然科学基金;

关键词：

Bacterial infection; Injectable hydrogel; Probiotics;

D O I：

10.1016/j.ijbiomac.2024.135302

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methicillin-resistant Staphylococcus aureus (MRSA) infections pose great challenges to skin wound care due to the severe drug resistance developed in the clinic. There is an urgent need to exploit next-generation bactericidal therapeutics that are both antibiotic-free and multifunctional for enhanced wound healing. Herein, we designed a Ca2+-crosslinked alginate hydrogel (EcNSIN@Alg) containing two naturally derived bioactive components, probiotics Escherichia coli Nissle1917 (EcN) and Squid ink nanoparticles (SIN), to treat MRSA-infected wounds. The injectable composite hydrogel displayed excellent biocompatibility, photothermal antibacterial activity, and reactive oxygen species (ROS) scavenging property. Importantly, the probiotic EcN can enhance the photothermal SIN to promote immune regulatory activities, shifting pro-inflammatory macrophages (M1) to antiinflammatory macrophages (M2). In an MRSA-infected abscess model, EcNSIN@Alg can reduce the expression level of wound inflammatory factors and ROS, increase the number of anti-inflammatory macrophages, accelerate collagen deposition and promote wound healing. This work offers a new perspective on developing safe, antibiotic-free, multifunctional bactericides using fully bioderived materials, with potential applications in clinical practice.

引用

页数：13