Anti-Inflammatory Activities of Yataprasen Thai Traditional Formulary and Its Active Compounds, Beta-Amyrin and Stigmasterol, in RAW264.7 and THP-1 Cells

Yataprasen (YTPS) remedy formulary, a national Thai traditional medicine formulary, comprises 13 herbal plants. It has been extensively prescribed to relieve osteoarthritis and musculoskeletal pain in the Thai traditional medicine healthcare system. The aim of this study was to investigate the antioxidant and anti-inflammatory properties of the bioactive compounds (beta-amyrin and stigmasterol) of YTPS remedy formulary ethanolic extract, along with its composition. The YTPS formulary extract contains 70.30 nM of beta-amyrin and 605.76 nM of stigmasterol. The YTPS formulary extract exhibited ABTS and DPPH free radical scavenging activity, with IC50 values of 144.50 +/- 2.82 and 31.85 +/- 0.18 mu g/mL, respectively. The ethanolic extract of YTPS at a concentration of 1000 mu g/mL showed a significant (p < 0.01) anti-inflammatory effect, mainly by reducing IL-6 and TNF-alpha release in response to LPS. NO production was prominently lowered by 50% at 24.76 +/- 1.48 <mu>g/mL, 55.52 +/- 24.40 mu M, and more than 570 mu M of YTPS formulary extract, beta-amyrin, and stigmasterol, respectively. Major components of YTPS, beta-amyrin, and stigmasterol exerted significant anti-inflammatory effects by inhibiting LPS-induced IL-1 beta, IL-6, TNF-alpha secretion in THP-1 cells. Our findings suggest that the ethanolic extract from YTPS holds promise as an alternative topical treatment for osteoarthritis and inflammatory disorders, potentially with fewer side effects than non-steroidal anti-inflammatory medications (NSAIDs).