Blockade of ITGA2/3/5 Promotes Adipogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells

被引：0

作者：

Li, Ying ^{[1
]}

Wang, Wendi ^{[1
]}

Liu, Zijian ^{[1
]}

Liu, Guangjing ^{[1
]}

Li, Xiaobing ^{[1
]}

机构：

[1] Tianjin First Cent Hosp, Dept Plast & Burn Surg, Tianjin, Peoples R China

来源：

CELL BIOCHEMISTRY AND BIOPHYSICS | 2025年 / 83卷 / 01期

关键词：

ITGA2/3/5; differentiation; hADSCs; adipocytes; Rac-1; pathway; INTEGRINS;

D O I：

10.1007/s12013-024-01545-w

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The integrin alpha (ITGA) subfamily genes play a fundamental role in various cancers. However, the potential mechanism and application values of ITGA genes in adipogenic differentiation of human adipose-derived stem cells (hADSCs) remain elusive. This study confirmed that ITGA2/3/5 mRNA expressions were repressed during adipogenesis. Blockade of ITGA2/3/5 enhanced adipogenic differentiation of hADSCs. Oil red O staining found that more lipid droplets were apparent in the ITGA2/3/5 inhibition group following 14 d adipogenic induction than in the control group. In addition, inhibition of ITGA2/3/5 promoted the expression of adipogenesis-related genes (PPAR-gamma, C/EBP alpha, FABP4). Mechanistically, ITGA2/3/5 functioned by regulating the Rac1 signaling pathway, which reasonably explains ITGA2/3/5's role in adipogenic differentiation of hADSCs. Our studies suggest that blockades of ITGA2/3/5 promote the adipogenic differentiation of hADSCs.

引用

页码：1105 / 1111

页数：7

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