Extracellular vesicle and lipoprotein diagnostics (ExoLP-Dx) with membrane sensor: A robust microfluidic platform to overcome heterogeneity

被引:2
作者
Kumar, Sonu [1 ]
Senapati, Satyajyoti [1 ]
Chang, Hsueh-Chia [1 ]
机构
[1] Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA
关键词
LOW-DENSITY-LIPOPROTEIN; PANCREATIC-CANCER; IMMUNE-RESPONSE; INTERCELLULAR COMMUNICATION; FLOW-CYTOMETRY; AMINO-ACIDS; EXOSOMES; BIOGENESIS; CELLS; NANOPARTICLES;
D O I
10.1063/5.0218986
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The physiological origins and functions of extracellular vesicles (EVs) and lipoproteins (LPs) propel advancements in precision medicine by offering non-invasive diagnostic and therapeutic prospects for cancers, cardiovascular, and neurodegenerative diseases. However, EV/LP diagnostics (ExoLP-Dx) face considerable challenges. Their intrinsic heterogeneity, spanning biogenesis pathways, surface protein composition, and concentration metrics complicate traditional diagnostic approaches. Commonly used methods such as nanoparticle tracking analysis, enzyme-linked immunosorbent assay, and nuclear magnetic resonance do not provide any information about their proteomic subfractions, including active proteins/enzymes involved in essential pathways/functions. Size constraints limit the efficacy of flow cytometry for small EVs and LPs, while ultracentrifugation isolation is hampered by co-elution with non-target entities. In this perspective, we propose a charge-based electrokinetic membrane sensor, with silica nanoparticle reporters providing salient features, that can overcome the interference, long incubation time, sensitivity, and normalization issues of ExoLP-Dx from raw plasma without needing sample pretreatment/isolation. A universal EV/LP standard curve is obtained despite their heterogeneities.
引用
收藏
页数:16
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