Engineered Cell Elongation Promotes Extracellular Electron Transfer of Shewanella Oneidensis

被引：6

作者：

Li, Feng ^{[1
,2
]}

Yu, Huan ^{[1
,2
]}

Zhang, Baocai ^{[1
,2
]}

Hu, Chaoning ^{[1
,2
]}

Lan, Fei ^{[1
,2
]}

Wang, Yuxuan ^{[1
,2
]}

You, Zixuan ^{[1
,2
]}

Liu, Qijing ^{[1
,2
]}

Tang, Rui ^{[1
,2
]}

Zhang, Junqi ^{[1
,2
]}

Li, Chao ^{[1
,2
]}

Shi, Liang ^{[3
]}

Li, Wen-Wei ^{[4
]}

Nealson, Kenneth H. ^{[5
]}

Liu, Zhanying ^{[6
,7
]}

Song, Hao ^{[1
,2
,8
]}

机构：

[1] Tianjin Univ, Frontier Sci Ctr Synthet Biol, Minist Educ, Key Lab Syst Bioengn, Tianjin 300072, Peoples R China

[2] Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China

[3] China Univ Geosci Wuhan, Dept Biol Sci & Technol, Sch Environm Studies, Wuhan 430074, Hubei, Peoples R China

[4] Univ Sci & Technol China, Chinese Acad Sci, Key Lab Urban Pollutant Convers, Dept Environm Sci & Engn, Hefei 230026, Peoples R China

[5] Univ Southern Calif, Dept Earth Sci & Biol Sci, 4953 Harriman Ave, South Pasadena, CA 91030 USA

[6] Inner Mongolia Univ Technol, Ctr Energy Conservat & Emiss Reduct Fermentat Ind, Engn Res Ctr Inner Mongolia Green Mfg Biofermentat, Hohhot 010051, Inner Mongolia, Peoples R China

[7] Inner Mongolia Univ Technol, Sch Chem Engn, Hohhot 010051, Inner Mongolia, Peoples R China

[8] Haihe Lab Sustainable Chem Transformat, Tianjin 300192, Peoples R China

来源：

ADVANCED SCIENCE | 2024年 / 11卷 / 41期

基金：

中国国家自然科学基金;

关键词：

biofilm formation; cellular length; c-type cytochromes; divisome; extracellular electron transfer (EET); ESCHERICHIA-COLI; ENHANCED BIOSYNTHESIS; TRANSPORT; MORPHOLOGY; BACTERIA; FLAVINS; METABOLISM; MECHANISMS; REVEALS; SYSTEM;

D O I：

10.1002/advs.202403067

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To investigate how cell elongation impacts extracellular electron transfer (EET) of electroactive microorganisms (EAMs), the division of model EAM Shewanella oneidensis (S. oneidensis) MR-1 is engineered by reducing the formation of cell divisome. Specially, by blocking the translation of division proteins via anti-sense RNAs or expressing division inhibitors, the cellular length and output power density are all increased. Electrophysiological and transcriptomic results synergistically reveal that the programmed cell elongation reinforces EET by enhancing NADH oxidation, inner-membrane quinone pool, and abundance of c-type cytochromes. Moreover, cell elongation enhances hydrophobicity due to decreased cell-surface polysaccharide, thus facilitates the initial surface adhesion stage during biofilm formation. The output current and power density all increase in positive correction with cellular length. However, inhibition of cell division reduces cell growth, which is then restored by quorum sensing-based dynamic regulation of cell growth and elongation phases. The QS-regulated elongated strain thus enables a cell length of 143.6 +/- 40.3 mu m (72.6-fold of that of S. oneidensis MR-1), which results in an output power density of 248.0 +/- 10.6 mW m(-2 )(3.41-fold of that of S. oneidensis MR-1) and exhibits superior potential for pollutant treatment. Engineering cellular length paves an innovate avenue for enhancing the EET of EAMs.

引用

页数：14

共 95 条

[1] Biphasic growth dynamics control cell division in Caulobacter crescentus [J].