Phenolic profiling by UPLC-MS/MS and hepatoprotective activity of Cardiospermum halicacabum against CCl4 induced liver injury in Wistar rats

The hepatoprotective potential of ethanolic extract of Cardiospermum halicacabum (ECH) was evaluated in Wistar rats having liver injury induced by CCl4. The free radical scavenging activity of ECH was evaluated by ABTS and DPPH assay. Phenolic compounds like luteolin 7-o-glucoronide, apigenin 7-o-glucoronide and chrysoerisol 7-o-glucoronide were identified in ECH by UPLC-MS/MSn analysis. On the other hand, the anti mutagenic effect of ECH was examined using tester strain Salmonella typhimurium TA100 with and without S9 extract. CCl4 administered Wistar rats exhibited alteration in various biochemical parameters which were significantly (P <= 0.05) restored toward normal level by the therapy of ECH. ECH significantly (P <= 0.05) reduced LPO level in the liver tissue. Treatment with ECH showed curative effect on histopathological alterations and DNA damage in CCl4 administered rats in a dose dependent manner. Silymarin, a routine hepatoprotective drug was used as reference standard. (C) 2012 Elsevier Ltd. All rights reserved.