Comparative transcriptome analysis of whiteflies raised on cotton leaf curl Multan virus-infected cotton plants

被引:2
作者
Chen, Ting [1 ]
Jia, Yanbo [1 ]
Chen, Jie [1 ]
Qi, Guojun [1 ]
机构
[1] Guangdong Acad Agr Sci, Guangdong Prov Key Lab High Technol Plant Protect, Plant Protect Res Inst, Guangzhou, Peoples R China
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
cotton leaf curl Multan virus; Bemisia tabaci; cryptic species; transcriptome; expression profiles; BEMISIA-TABACI; TRANSMISSION; BEGOMOVIRUSES; EXPRESSION; GENES; ACQUISITION; DEFENSE; DISEASE; BARRIER; GENOME;
D O I
10.3389/fvets.2024.1417590
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Cotton leaf curl Multan virus (CLCuMuV), a serious viral disease causative agent in cotton plants in South Asia, is transmitted by the Bemisia tabaci cryptic species complex in a persistent circulative manner. A previous study indicated that Asia II-7 whiteflies could transmit CLCuMuV, while Mediterranean (MED) whiteflies failed to transmit CLCuMuV. However, little is known about the genes involved in this process. In this study, Asia II-7 and MED B. tabaci were utilized to determine transcriptomic responses after 48 h of acquisition access periods (AAPs). Result of Illumina sequencing revealed that, 14,213 and 8,986 differentially expressed genes (DEGs) were identified. Furthermore, DEGs related to the immune system and metabolism of Asia II-7 and MED in response to CLCuMuV-infected plants were identified and analyzed using Gene Ontologies (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG), and the number of related DEGs in MED was lower than that of Asia II-7. The most abundant groups of DEGs between both viruliferous and aviruliferous whitefly species were the zf-C2H2 family of transcription factors (TFs). Notably, in comparison to viruliferous MED, Asia II-7 exhibited more DEGs related to cathepsin biosynthesis. Overall, this study provides the basic information for investigating the molecular mechanism of how begomoviruses affect B. tabaci metabolism and immune response either as vector cryptic species or non-vector species.
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页数:13
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