The Effect of the Lysine Acetylation Modification of ClpP on the Virulence of Vibrio alginolyticus

被引:0
作者
Wang, Shi [1 ,2 ]
Jiang, Yingying [1 ,2 ]
Zhang, Weijie [1 ,2 ]
Wei, Yingzhu [1 ,2 ]
Xiao, Xing [1 ,2 ]
Wei, Zhiqing [1 ,2 ]
Wen, Xiaoxin [1 ,2 ]
Dong, Yuhang [1 ,2 ]
Jian, Jichang [1 ,2 ]
Wang, Na [3 ]
Pang, Huanying [1 ,2 ]
机构
[1] Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524025, Peoples R China
[2] Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524025, Peoples R China
[3] Chinese Acad Inspect & Quarantine, Beijing 100176, Peoples R China
来源
MOLECULES | 2024年 / 29卷 / 17期
基金
中国国家自然科学基金;
关键词
Vibrio alginolyticus; ClpP; lysine acetylation; virulence; ALKALINE SERINE-PROTEASE; TYPHIMURIUM; EXPRESSION; GROUPER;
D O I
10.3390/molecules29174278
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Acetylation modification has become one of the most popular topics in protein post-translational modification (PTM) research and plays an important role in bacterial virulence. A previous study indicated that the virulence-associated caseinolytic protease proteolytic subunit (ClpP) is acetylated at the K165 site in Vibrio alginolyticus strain HY9901, but its regulation regarding the virulence of V. alginolyticus is still unknown. We further confirmed that ClpP undergoes lysine acetylation (Kace) modification by immunoprecipitation and Western blot analysis and constructed the complementation strain (C-clpP) and site-directed mutagenesis strains including K165Q and K165R. The K165R strain significantly increased biofilm formation at 36 h of incubation, and K165Q significantly decreased biofilm formation at 24 h of incubation. However, the acetylation modification of ClpP did not affect the extracellular protease (ECPase) activity. In addition, we found that the virulence of K165Q was significantly reduced in zebrafish by in vivo injection. To further study the effect of lysine acetylation on the pathogenicity of V. alginolyticus, GS cells were infected with four strains, namely HY9901, C-clpP, K165Q and K165R. This indicated that the effect of the K165Q strain on cytotoxicity was significantly reduced compared with the wild-type strain, while K165R showed similar levels to the wild-type strain. In summary, the results of this study indicate that the Kace of ClpP is involved in the regulation of the virulence of V. alginolyticus.
引用
收藏
页数:15
相关论文
共 56 条
  • [1] Cellular functions of the ClpP protease impacting bacterial virulence
    Aljghami, Mazen E.
    Barghash, Marim M.
    Majaesic, Emily
    Bhandari, Vaibhav
    Houry, Walid A.
    [J]. FRONTIERS IN MOLECULAR BIOSCIENCES, 2022, 9
  • [2] Effects of four antibiotics on Pseudomonas aeruginosa motility, biofilm formation, and biofilm-specific antibiotic resistance genes expression
    Arslan, Elif
    Coskun, Muhammed K.
    Cobanoglu, Seymanur
    Aslan, Mehtap H.
    Yazici, Aysenur
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2023, 106 (03)
  • [3] Vibrios as causal agents of zoonoses
    Austin, B.
    [J]. VETERINARY MICROBIOLOGY, 2010, 140 (3-4) : 310 - 317
  • [4] Regulation of bacterial physiology by lysine acetylation of proteins
    Bernal, Vicente
    Castano-Cerezo, Sara
    Gallego-Jara, Julia
    Ecija-Conesa, Ana
    de Diego, Teresa
    Luis Iborra, Jose
    Canovas, Manuel
    [J]. NEW BIOTECHNOLOGY, 2014, 31 (06) : 586 - 595
  • [5] Cloning and expression of the gene encoding an extracellular alkaline serine protease from Vibrio alginolyticus strain HY9901, the causative agent of vibriosis in Lutjanus erythopterus (Bloch)
    Cai, S. H.
    Wu, Z. H.
    Jian, J. C.
    Lu, Y. S.
    [J]. JOURNAL OF FISH DISEASES, 2007, 30 (08) : 493 - 500
  • [6] Construction and evaluation of Vibrio alginolyticus ΔclpP mutant, as a safe live attenuated vibriosis vaccine
    Chen, Yanyan
    Wu, Fenglei
    Wang, Zhiwen
    Tang, Jufen
    Cai, Shuanghu
    Jian, Jichang
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2020, 98 : 917 - 922
  • [7] Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions
    Choudhary, Chunaram
    Kumar, Chanchal
    Gnad, Florian
    Nielsen, Michael L.
    Rehman, Michael
    Walther, Tobias C.
    Olsen, Jesper V.
    Mann, Matthias
    [J]. SCIENCE, 2009, 325 (5942) : 834 - 840
  • [8] Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum
    Crabbe, Aurelie
    Liu, Yulong
    Matthijs, Nele
    Rigole, Petra
    De La Fuente-Nunez, Cesar
    Davis, Richard
    Ledesma, Maria A.
    Sarker, Shameema
    Van Houdt, Rob
    Hancock, Robert E. W.
    Coenye, Tom
    Nickerson, Cheryl A.
    [J]. SCIENTIFIC REPORTS, 2017, 7
  • [9] The Bacterial Phosphoenolpyruvate:Carbohydrate Phosphotransferase System: Regulation by Protein Phosphorylation and Phosphorylation-Dependent Protein-Protein Interactions
    Deutscher, Josef
    Ake, Francine Moussan Desiree
    Derkaoui, Meriem
    Zebre, Arthur Constant
    Thanh Nguyen Cao
    Bouraoui, Houda
    Kentache, Takfarinas
    Mokhtari, Abdelhamid
    Milohanic, Eliane
    Joyet, Philippe
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2014, 78 (02) : 231 - 256
  • [10] Glutathione Fine-Tunes the Innate Immune Response toward Antiviral Pathways in a Macrophage Cell Line Independently of Its Antioxidant Properties
    Diotallevi, Marina
    Checconi, Paola
    Palamara, Anna Teresa
    Celestino, Ignacio
    Coppo, Lucia
    Holmgren, Arne
    Abbas, Kahina
    Peyrot, Fabienne
    Mengozzi, Manuela
    Ghezzi, Pietro
    [J]. FRONTIERS IN IMMUNOLOGY, 2017, 8