Structural characteristics of a polysaccharide from Armillariella tabescens and its protective effect on colitis mice via regulating gut microbiota and intestinal barrier function

A new polysaccharide fraction (ATP) was obtained from Armillariella tabescens mycelium. Structural analysis suggested that the backbone of ATP was-*4)-alpha-D-Glcp(1 p (1-* 2)-alpha-D-Galp(1 p (1-* 2)-alpha-D-Glcp(1 p (1-* 4)-alpha-D-Glcp(1-*, p (1-*, which branched at O-3 of-*2)-alpha-D-Glcp(1 p (1-* and terminated with T-alpha-D-Glcp p or T-alpha-D-Manp. p . Besides, ATP significantly alleviated ulcerative colitis (UC) symptoms and inhibited the production of pro-inflammation cytokines (IL-1 beta, IL-6). Meanwhile, ATP could improve colon tissue damage by elevating the expression of MUC2 and tight junction proteins (ZO-1, occludin and claudin-1) levels and enhance intestinal barrier function through inhibiting the activation of MMP12/MLCK/p-MLC2 p-MLC2 signaling pathway. Further studies exhibited that ATP could increase the relative abundance of beneficial bacteria such as f. Muribaculacese, , g. Muribaculaceae, , and g. Alistips, , and decrease the relative abundance of g. Desulfovibrio, , g. Colidextribacter, , g. Ruminococcaceae and g .Oscillibacter , and regulate the level of short-chain fatty acids. Importantly, FMT intervention with ATP-derived microbiome certified that gut microbiota was involved in the protective effects of ATP on UC. The results indicated that ATP was potential to be further developed into promising therapeutic agent for UC.