FITC & TRITC Spot Detection and Analysis at BCL2 Gene Locus as Follicular Lymphoma Marker on 2D Digital Tissue Slides and 3D Serial Sections

被引：0
作者：
Kucarov, Marianna Dimitrova [1 ]
Csizmadia, Annamaria [2 ]
Molnar, Bela [3 ]
Kozlovszky, Miklos [4 ,5 ]
机构：
[1] Obuda Univ, 3DHistech Ltd, BioTech, Doctoral Sch Appl Informat & Appl Math, Budapest, Hungary
[2] Semmelweis Univ, Doctoral Sch Pathol Sci, Budapest, Hungary
[3] 3DHistech Ltd, Budapest, Hungary
[4] Obuda Univ, John von Neumann Fac Informat, H-1034 Bp, Hungary
[5] Hungarian Res Network HUN REN, Inst Comp Sci & Control SZTAKI, Med Device Res Grp, LPDS, Budapest, Hungary
来源：
28TH INTERNATIONAL CONFERENCE ON INTELLIGENT ENGINEERING SYSTEMS, INES 2024 | 2024年
关键词：
Follicular lymphoma; FISH; BCL2; gene; FITC and TRITC spot detection; serial section; digital slide visualization; EXPRESSION; IMAGE; FISH;
D O I：
10.1109/INES63318.2024.10629118
中图分类号：
TP18 [人工智能理论];
学科分类号：
081104 ; 0812 ; 0835 ; 1405 ;
摘要：
We aimed to automate the detection and measurement of chromosomal rearrangements at the BCL2 locus located on chromosome 18q21 in order to further refine the BCL2 based therapeutic strategies for follicular lyphoma and speed up its diagnostics. The implementation was carried out using fluorescence in situ hybridization, PANNORAMIC MIDI II fluorescence slide scanner, and digital image processing techniques. The method provides visualization of the evaluated digital tissue slides for 2D and 3D serial sections as well.
引用
收藏
页码：83 / 88
页数：6
相关论文
共 19 条
  • [1] 3DHistech Ltd, 2020, PANNORAMIC MIDI II
  • [2] Abbott, 2023, Vysis LSI BCL2 Break Apart FISH Probe kit
  • [3] Novel markers in pediatric-type follicular lymphoma
    Agostinelli, Claudio
    Akarca, Ayse U.
    Ramsay, Alan
    Rizvi, Hasan
    Rodriguez-Justo, Manuel
    Pomplun, Sabine
    Proctor, Ian
    Sabattini, Elena
    Linch, David
    Daw, Stephen
    Pittaluga, Stefania
    Pileri, Stefano A.
    Jaffe, Elaine S.
    Quintanilla-Martinez, Leticia
    Marafioti, Teresa
    [J]. VIRCHOWS ARCHIV, 2019, 475 (06) : 771 - 779
  • [4] Expression of inhibitor of apoptosis proteins in B-cell non-Hodgkin and Hodgkin lymphomas
    Akyurek, Nalan
    Ren, Yongsheng
    Rassidakis, Georgios Z.
    Schlette, Ellen J.
    Medeiros, L. Jeffrey
    [J]. CANCER, 2006, 107 (08) : 1844 - 1851
  • [5] Follicular lymphoma
    Carbone, Antonino
    Roulland, Sandrine
    Gloghini, Annunziata
    Younes, Anas
    von Keude, Gottfried
    Lopez-Guillermo, Armando
    Fitzgibbons, Jude
    [J]. NATURE REVIEWS DISEASE PRIMERS, 2019, 5 (1)
  • [6] Csizmadia A, 2022, VIRCHOWS ARCH, V481, pS303
  • [7] Csizmadia A., 2021, Medicina Konyvkiado, P439
  • [8] Translocation (14;18)(q32;q21) in acute lymphoblastic leukemia: a study of 12 cases and review of the literature
    D'Achille, Pina
    Seymour, John F.
    Campbell, Lynda J.
    [J]. CANCER GENETICS AND CYTOGENETICS, 2006, 171 (01) : 52 - 56
  • [9] Follicular lymphoma: 2020 update on diagnosis and management
    Freedman, Arnold
    Jacobsen, Eric
    [J]. AMERICAN JOURNAL OF HEMATOLOGY, 2020, 95 (03) : 316 - 327
  • [10] Fluorescence in situ hybridization (FISH) in the microbiological diagnostic routine laboratory: a review
    Frickmann, Hagen
    Zautner, Andreas Erich
    Moter, Annette
    Kikhney, Judith
    Hagen, Ralf Matthias
    Stender, Henrik
    Poppert, Sven
    [J]. CRITICAL REVIEWS IN MICROBIOLOGY, 2017, 43 (03) : 263 - 293
12