Differential responses in water-use strategies of evergreen ( Ligustrum lucidum) ) and deciduous ( Robinia pseudoacacia) ) trees to tunnel excavation in a subtropical karst trough valley

Tunnel(s) excavation could decrease groundwater levels and soil water storage and consequently change trees growth conditions in karst areas. However, the adaptation mechanisms of different tree species to altered hydrological conditions caused by tunnel(s) construction in karst areas remain unknown. Here, we investigated the water-use strategies of two different tree species (the evergreen Ligustrum lucidum and deciduous Robinia pseudoacacia) in response to tunnel excavation in a typical subtropical karst trough valley by using the sap flow density, stable isotopes (S13C, S2H, and S18O) and MixSIAR models. Tunnel excavation resulted in a 3.7-6.3 % reduction in soil moisture content (SMC) in Zhongliang Mountain Karst Trough (ZMKT) Valley. Both tree species demonstrated reduced transpiration in tunnel-affected valley (TAV) compared to tunnel-unaffected valley (TUAV) (transpiration in TAV was only 77.6 % of TUAV), with evergreen trees exhibiting a smaller decline in transpiration compared to deciduous trees. Furthermore, the proportions of karst groundwater utilized by evergreen and deciduous tree species in TAV were 8.3 % and 5.0 % higher than in TUAV, respectively, with evergreen tree species exhibiting greater absorption of karst groundwater than deciduous tree species. Meanwhile, the average leaf S13C values and WUEi (intrinsic water-use efficiency) of both tree species in TAV were 0.9 parts per thousand and 15.7 mu mol/mol higher than those in TUAV, respectively, with evergreen trees exhibiting a smaller increase in WUEi compared to deciduous trees. These results indicate that tunneling-induced water stress events may prompt trees to adopt more conservative water-use strategies. Evergreen tree species rely on more karst groundwater absorption to sustain relatively higher transpiration rates, and deciduous trees adapt to severe water stress by increasing their WUEi or shedding leaves to minimize transpiration. Our finding contributes to the evaluation of eco-hydrological processes in karst areas affected by tunnel(s) excavation.