Deciphering retention effect of extracellular polymeric substances to typical heavy metals and their interaction with key inner enzymes of Candidatus Kuenenia

被引:2
作者
He, Yuhang [1 ]
Jiang, Zhicheng [1 ]
Zeng, Ming [1 ]
Cao, Shenbin [2 ,3 ]
Yu, Xiaohui [4 ]
Wu, Nan [4 ]
机构
[1] Tianjin Univ Sci & Technol, Coll Marine & Environm Sci, Tianjin 300457, Peoples R China
[2] Beijing Univ Technol, Engn Res Ctr Beijing, Natl Engn Lab Adv Municipal Wastewater Treatment &, Beijing 100124, Peoples R China
[3] Beijing Univ Technol, Coll Architecture & Civil Engn, Fac Architecture Civil & Transportat Engn FACTE, Beijing 100124, Peoples R China
[4] Tianjin Agr Univ, Coll Engn & Technol, Tianjin 300384, Peoples R China
基金
中国国家自然科学基金;
关键词
Anammox; Heavy metals; EPS; Functional genes; Molecular simulation; ANAMMOX ACTIVITY; INHIBITION; RECOVERY; EPS; MECHANISM; BATCH; ZINC;
D O I
10.1016/j.jhazmat.2024.135367
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
This study employed spectroscopy, metagenomics, and molecular simulation to investigate the inhibitory effects of Cd(II) and Cu(II) on the anammox system, examining both intracellular and extracellular effects. At concentrations of 5 mg/L, Cd(II) and Cu(II) significantly reduced nitrogen removal efficiency by 41.46 % and 62.03 %, respectively. Additionally, elevated metal concentrations were correlated with decreased extracellular polymeric substances (EPS), thereby reducing their capacity to absorb heavy metals, particularly Cu(II), which decreased from 76.47 % to 14.67 %. Spectral analysis revealed alterations in the secondary structures of EPS induced by Cd(II) and Cu(II), decreasing the ratio of extracellular protein alpha-helix to (R-sheet + random coil), which resulted in looser extracellular protein configurations. The results of the metagenomics study showed that the abundance of Candidatus Kuenenia and its genes encoding nitrogen removal-related enzymes was reduced. The abundance of hzs-gamma was reduced by 35.09 % at a concentration of 5 mg/L Cu(II). Conversely, genes associated with metal efflux enzymes, like czcR, increased by 54.86 % at 2 mg/L Cd(II). Molecular docking revealed robust bindings of Cd(II) to HZS-alpha (-342.299 f 218.165 kJ/mol) and Cu(II) to HZS-gamma (-880.934 f 55.526 kJ/ mol). This study elucidated the inhibitory mechanisms of Cd(II) and Cu(II) on the anammox system, providing insights into the resistance of anammox bacteria to heavy metals.
引用
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页数:10
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