Evaluation of Bioactive Compounds and Antioxidant and Cytotoxic Effects of Oil and Pulp without Acai Fat (Euterpe oleracea) Obtained by Supercritical Extraction

被引:0
作者
Siqueira, Leticia Maria Martins [1 ]
de Barros Souza Campos, Ana Luiza [2 ]
Pires, Flavia Cristina Seabra [3 ]
Ferreira, Maria Caroline Rodrigues [4 ]
de Souza e Silva, Ana Paula [4 ]
Menezes, Eduardo Gama Ortiz [5 ]
de Farias Ramos, Ingryd Nayara [6 ]
Khayat, Andre Salim [7 ]
de Arimateia Rodrigues do Rego, Jose [8 ]
de Carvalho Jr, Raul Nunes [9 ]
机构
[1] UFPA Fed Univ Para, ITEC Inst Technol, LABTECS Supercrit Technol Lab, PRODERNA Grad Program Engn Nat Resources Amazon,LA, Augusto Correa St S-N, BR-66075900 Belem, PA, Brazil
[2] UFPA Fed Univ Para, ITEC Inst Technol, FEQ Fac Chem Engn, LABEX Extract Lab, Rua Augusto Correa S-N, BR-66075900 Belem, PA, Brazil
[3] LABTECS Supercrit Technol Lab, Sci & Technol Pk,Perimeter Ave Sci,Km 01, BR-66075750 Belem, PA, Brazil
[4] UFPA Fed Univ Para, ITEC Inst Technol, PPGCTA Grad Program Food Sci & Technol, LABEX Extract Lab,LABTECS Supercrit Technol Lab, Augusto Correa St S-N, BR-66075900 Belem, PA, Brazil
[5] Fed Inst Educ Sci & Technol Rondonia, IFRO, BR-78900000 Porto Velho, RO, Brazil
[6] UFPA Fed Univ Para, ICB Inst Biol Sci, NPO Ctr Res Oncol, PPGOCM Grad Program Oncol & Med Sci, Augusto Correa St S-N,, BR-66507900 Belem, PA, Brazil
[7] UFPA Fed Univ Para, ICB Inst Biol Sci, NPO Oncol Res Ctr, Augusto Correa St S-N, BR-66075900 Belem, PA, Brazil
[8] UFPA Fed Univ Para, ICEN Inst Exact & Nat Sci, PPGCMA Grad Program Sci & Environm, LABA Lab Amazonian Biofilms, Augusto Correa St S CON, BR-66507900 Belem, PA, Brazil
[9] UFPA Fed Univ Para, ITEC Inst Technol, FEA Fac Food Engn, LABEX Extract Lab,LABTECS Supercrit Technol Lab, Augusto Correa St S-N, BR-66507900 Belem, PA, Brazil
关键词
supercritical CO2; oil extraction; green process; anthocyanins; carotenoids; fat-free acai; FLUID EXTRACTION; CO2; EXTRACTION; MART; ANTHOCYANINS; CHEMISTRY; CAPACITY; YIELD; RISK;
D O I
10.3390/foods13172819
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
For bioactivity studies, it is necessary to use products with a high degree of purity, which may influence the cytotoxic effects. Supercritical technology presents itself as an alternative to obtain these products. Therefore, the objective of this work was to obtain the bioactive compounds of oil and pulp of a & ccedil;a & iacute; fat-free supercritical technology and evaluate the cytotoxicity of products in MRC-5 and VERO cells in vitro. The a & ccedil;a & iacute; pulp was subjected to extraction with supercritical CO2 to obtain the oil and pulp without fat, under conditions of 323.15 K at 35 MPa, 333.15 K at 42 MPa, and 343.15 K at 49 MPa. The largest yields (51.74%), carotenoids (277.09 mu g/g), DPPH (2.55 mu mol TE/g), ABTS (2.60 mu mol TE/g), and FRAP (15.25 mu m of SF/g) of oil and ABTS (644.23 mu mol TE/g) of pulp without fat were found in the condition 343.15 K at 49 MPa. The highest levels of compounds phenolics (150.20 mg GAE/g), DPPH (414.99 mu mol TE/g), and FRAP (746.2 mu m SF/g) of the pulp without fat were found in the condition of 323.15 K to 35 MPa. The fat-free pulp presented high levels of anthocyanins without significant variation in cytotoxicity. The developed process was efficient in obtaining oil rich in carotenoids, and the supercritical technology elucidated an efficient way to obtain acai fat-free pulp.
引用
收藏
页数:18
相关论文
共 95 条
[11]   Food sustainability trends-How to value the acai production chain for the development of food inputs from its main bioactive ingredients? [J].
Barbosa, Jhonatas Rodrigues ;
de Carvalho Junior, Raul Nunes .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2022, 124 :86-95
[12]   Global warming effects on yield and fruit maturation of olive trees growing under field conditions [J].
Benlloch-Gonzalez, Maria ;
Sanchez-Lucas, Rosa ;
Aymen Bejaoui, Mohamed ;
Benlloch, Manuel ;
Fernandez-Escobar, Ricardo .
SCIENTIA HORTICULTURAE, 2019, 249 :162-167
[13]   The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].
Benzie, IFF ;
Strain, JJ .
ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76
[14]   The Benefits and Risks of Certain Dietary Carotenoids that Exhibit both Anti- and Pro-Oxidative Mechanisms-A Comprehensive Review [J].
Black, Homer S. ;
Boehm, Fritz ;
Edge, Ruth ;
Truscott, T. George .
ANTIOXIDANTS, 2020, 9 (03)
[15]   Chemical and sensorial characterization of a novel alcoholic beverage produced with native acai (Euterpe precatoria) from different regions of the Amazonas state [J].
Boeira, Lucia S. ;
Bastos Freitas, Paulo H. ;
Uchoa, Nicole R. ;
Bezerra, Jaqueline A. ;
Cad, Sandra, V ;
Duvoisin Junior, Sergio ;
Albuquerque, Patricia M. ;
Mar, Josiana M. ;
Ramos, Andrezza S. ;
Machado, Marcos B. ;
Maciel, Luis R. .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2020, 117
[16]   Phenolic compounds from coffee by-products: Extraction and application in the food and pharmaceutical industries [J].
Bondam, Aline Felten ;
da Silveira, Daiele Diolinda ;
dos Santos, Jaqueline Pozzada ;
Hoffmann, Jessica Fernanda .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2022, 123 :172-186
[17]   Bacaba-de-leque (Oenocarpus distichus Mart.) oil extraction using supercritical CO2 and bioactive compounds determination in the residual pulp [J].
Borges Cunha, Vania Maria ;
da Silva, Marcilene Paiva ;
Brabo de Sousa, Sergio Henrique ;
Bezerra, Priscila do Nascimento ;
Ortiz Menezes, Eduardo Gama ;
Neves da Silva, Nayara Janaina ;
Dornelas da Silva Banna, Debora Ariane ;
Araujo, Marilena Emmi ;
de Carvalho Junior, Raul Nunes .
JOURNAL OF SUPERCRITICAL FLUIDS, 2019, 144 :81-90
[18]  
BRAND-WILLIAMS W, 1995, FOOD SCI TECHNOL-LEB, V28, P25
[19]   Protective effect of Euterpe edulis M. on Vero cell culture and antioxidant evaluation based on phenolic composition using HPLC - ESI-MS/MS [J].
Campelo Borges, Graciele Da Silva ;
Gonzaga, Luciano Valdemiro ;
Jardini, Fernanda Archilla ;
Mancini Filho, Jorge ;
Heller, Melina ;
Micke, Gustavo ;
Oliveira Costa, Ana Carolina ;
Fett, Roseane .
FOOD RESEARCH INTERNATIONAL, 2013, 51 (01) :363-369
[20]  
Cavalcante P., 2018, Braz. J. Food Technol, V21, DOI [10.1590/1981-6723.09217, DOI 10.1590/1981-6723.09217]