Characterization of Amniotic Fluid Ureaplasma Species from Pregnancies Complicated by Preterm Prelabor Rupture of Membranes

被引:0
作者
Libra, Antonin [1 ,2 ]
Bolehovska, Radka [3 ,4 ]
Kukla, Rudolf [3 ,4 ]
Musilova, Ivana [1 ,4 ,5 ]
Menon, Ramkumar [6 ]
Jacobsson, Bo [7 ,8 ,9 ]
Kacerovsky, Marian [1 ,4 ,5 ]
机构
[1] Univ Hosp Hradec Kralove, Biomed Res Ctr, Sokolska 581, Hradec Kralove 50005, Czech Republic
[2] Generi Biotech, Hradec Kralove, Czech Republic
[3] Univ Hosp, Inst Clin Microbiol, Hradec Kralove, Czech Republic
[4] Charles Univ Prague, Inst Clin Microbiol, Med Fac Hradec Kralove, Hradec Kralove, Czech Republic
[5] Krajska Zdravotni A s, Hosp Most, Dept Obstet & Gynecol, Most, Czech Republic
[6] Univ Texas Galveston, Dept Obstet & Gynecol, Div Basic Sci & Translat Res, Med Branch, Galveston, TX USA
[7] Univ Gothenburg, Inst Clin Sci, Sahlgrenska Acad, Dept Obstet & Gynecol, Gothenburg, Sweden
[8] Sahlgrens Univ Hosp, Dept Obstet & Gynecol, Reg Vastra Gotaland, Gothenburg, Sweden
[9] Inst Publ Hlth, Dept Genet & Bioinformat, Domain Hlth Data & Digitalisat, Oslo, Norway
关键词
Microbial invasion of the amniotic cavity; Genital mycoplasma; Molecular biology; Mollicutes; Morbidity; Neonates; Preterm delivery; Sequencing; PREMATURE RUPTURE; UREALYTICUM; INFECTION;
D O I
10.1007/s43032-024-01697-3
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The main aim of this study was to determine expanded sequence types (eSTs) of Ureaplasma species (U. spp.). DNA isolated from the amniotic fluid of pregnancies complicated by preterm prelabor rupture of membranes (PPROM) using an expanded multilocus sequence typing scheme. Additionally, the study sought to examine whether phylogenetic subgroups of U. spp. DNA differ with respect to maternal demographic and clinical parameters and selected aspects of short-term neonatal morbidity. This retrospective cohort study was focused on singleton pregnancies complicated by PPROM occurring between the gestational ages of 24+0 and 36+6 weeks, where amniocentesis was conducted to assess the intra-amniotic environment and the presence of U. spp. DNA in the amniotic fluid samples was confirmed. The stored aliquots of U. spp. DNA were used to assess differences in nucleotide sequences in six U. spp. genes (ftsH, rpL22, valS, thrS,ureG, and mba-np1) using the eMLST scheme. The expanded multilocus sequence typing scheme was performed in 73 samples of U. spp. DNA isolated from pregnancies complicated by PPROM. In total, 33 different U. spp. DNA eSTs were revealed, 21 (#20, 233-244, 248-251, 253, 255, 259, and 262) of which were novel. The most frequently identified eST was #41, identified in 18% (13/73) of the aliquots. Based on their genetic relationships, the U. spp. DNA was divided into two clusters and four subgroups [cluster I (U. parvum): A, 43% (n = 31); B, 15% (n = 11); and C, 26% (n = 19); cluster II (U. urealyticum): 1; 16% (n = 12)]. Cluster II had a higher rate of polymicrobial findings than cluster I (58% vs 16%; p = 0.005), while subgroup A had the highest rate of concomitant Mycoplasma hominis in the amniotic fluid samples (66%; p = 0.04). In conclusion, Ureaplasma spp. DNA obtained from PPROM consisted of 33 different eSTs of U. spp. DNA. No differences in maternal and neonatal characteristics were found among the phylogenetical subgroups of U. spp. DNA, except for a higher rate of polymicrobial amniotic fluid findings in those with U. urealyticumand the concomitant presence of M. hominis in the amniotic fluid in those with the presence of U. parvum.
引用
收藏
页码:3440 / 3451
页数:12
相关论文
共 39 条
[1]   Multilocus sequence typing characterizes diversity of Ureaplasma diversum strains, and intra-species variability induces different immune response profiles [J].
Andrade, Yasmin M. F. S. ;
Santos-Junior, Manoel N. ;
Rezende, Izadora S. ;
Barbosa, Maysa S. ;
Amorim, Aline T. ;
Silva, Icaro B. S. ;
Queiroz, Ellunny C. ;
Bastos, Bruno L. ;
Campos, Guilherme B. ;
Timenetsky, Jorge ;
Marques, Lucas M. .
BMC VETERINARY RESEARCH, 2020, 16 (01)
[2]   Preterm premature rupture of the membranes: current approaches to evaluation and management [J].
DiGiulio, Daniel B. ;
Romero, Roberto ;
Kusanovic, Juan Pedro ;
Gomez, Ricardo ;
Kim, Chong Jai ;
Seok, Kimberley S. ;
Gotsch, Francesca ;
Mazaki-Tovi, Shali ;
Vaisbuch, Edi ;
Sanders, Katherine ;
Bik, Elisabeth M. ;
Chaiworapongsa, Tinnakorn ;
Oyarzun, Enrique ;
Relman, David A. .
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2010, 64 (01) :38-57
[3]   Quantification of Ureaplasma urealyticum DNA in the amniotic fluid from patients in PTL and pPROM and its relation to inflammatory cytokine levels [J].
Jacobsson, Bo ;
Aaltonen, Riikka ;
Rantakokko-Jalava, Kaisu ;
Morken, Nils-Halvdan ;
Alanen, Anna .
ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, 2009, 88 (01) :63-70
[4]   Extreme preterm birth -: Onset of delivery and its effect on infant survival and morbidity [J].
Johanzon, Madelene ;
Odesjo, Helena ;
Jacobsson, Bo ;
Sandberg, Kenneth ;
Wennerholm, Ulla-Britt .
OBSTETRICS AND GYNECOLOGY, 2008, 111 (01) :42-50
[5]  
Jolley KA., 2018, WELLCOME OPEN RES, V3, DOI [10.12688/wellcomeopenres.14826.1, DOI 10.12688/WELLCOMEOPENRES.14826.1]
[6]   Prevalence and Load of Cervical Ureaplasma Species With Respect to Intra-amniotic Complications in Women With Preterm Prelabor Rupture of Membranes Before 34 weeks [J].
Kacerovsky, Marian ;
Kukla, Rudolf ;
Bolehovska, Radka ;
Bostik, Pavel ;
Matulova, Jana ;
Mls, Jan ;
Stranik, Jaroslav ;
Jacobsson, Bo ;
Musilova, Ivana .
FRONTIERS IN PHARMACOLOGY, 2022, 13
[7]   Clinical characteristics of colonization of the amniotic cavity in women with preterm prelabor rupture of membranes, a retrospective study [J].
Kacerovsky, Marian ;
Stranik, Jaroslav ;
Matulova, Jana ;
Chalupska, Martina ;
Mls, Jan ;
Faist, Tomas ;
Hornychova, Helena ;
Kukla, Rudolf ;
Bolehovska, Radka ;
Bostik, Pavel ;
Jacobsson, Bo ;
Musilova, Ivana .
SCIENTIFIC REPORTS, 2022, 12 (01)
[8]   Intra-amniotic infection and sterile intra-amniotic inflammation in women with preterm labor with intact membranes are associated with a higher rate of Ureaplasma species DNA presence in the cervical fluid [J].
Kacerovsky, Marian ;
Stranik, Jaroslav ;
Kukla, Rudolf ;
Bolehovska, Radka ;
Bostik, Pavel ;
Matulova, Jana ;
Stepan, Martin ;
Hladky, Jan ;
Jacobsson, Bo ;
Musilova, Ivana .
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE, 2022, 35 (25) :7344-7352
[9]   Amniotic Fluid Protein Profiles of Intraamniotic Inflammatory Response to Ureaplasma spp. and Other Bacteria [J].
Kacerovsky, Marian ;
Celec, Peter ;
Vlkova, Barbora ;
Skogstrand, Kristin ;
Hougaard, David M. ;
Cobo, Teresa ;
Jacobsson, Bo .
PLOS ONE, 2013, 8 (03)
[10]   The impact of the microbial load of genital mycoplasmas and gestational age on the intensity of intraamniotic inflammation [J].
Kacerovsky, Marian ;
Pliskova, Lenka ;
Bolehovska, Radka ;
Skogstrand, Kristin ;
Hougaard, David M. ;
Tsiartas, Panagiotis ;
Jacobsson, Bo .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2012, 206 (04) :342.e1-342.e8