Hepatic endotheliitis in Golden Syrian hamsters (Mesocricetus auratus) experimentally infected with SARS-CoV-2

被引:0
作者
de Souza, Alex Junior Souza [1 ]
de Souza, Antonio Francisco [2 ]
Zimpel, Cristina Kraemer [2 ,3 ]
Ayupe, Marina Cacador [4 ]
de Araujo, Marcelo Valdemir [2 ,4 ,5 ]
Machado, Rafael Rahal Guaragna [2 ]
Salles, Erika [4 ]
Salgado, Caio Loureiro [4 ]
Tavares, Mariana Silva [2 ]
Silva-Pereira, Taiana Taina [2 ]
de Souza, Paula Carolina [4 ]
Durigon, Edison Luiz [2 ]
Heinemann, Marcos Bryan [3 ]
Brandao, Paulo Eduardo [3 ]
da Fonseca, Denise Morais
Guimaraes, Ana Marcia de Sa [2 ]
de Sa, Lilian Rose Marques [1 ]
机构
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Ave Prof. Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP, Brazil
[2] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Sao Paulo, SP, Brazil
[3] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo, SP, Brazil
[4] Univ Sao Paulo, Dept Imunol, Inst Ciencias Biomed, Sao Paulo, SP, Brazil
[5] Inst Butantan, Lab Virol, Ctr Desenvolvimento & Inovacao, Sao Paulo, SP, Brazil
来源
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO | 2024年 / 66卷
基金
巴西圣保罗研究基金会;
关键词
Animal model; COVID-19; Endothelialitis; Hepatitis; Liver; HOST-DISEASE; LIVER; GRAFT;
D O I
10.1590/S1678-9946202466044
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Hepatic injuries in COVID-19 are not yet fully understood and indirect pathways (without viral replication in the liver) have been associated with the activation of vascular mechanisms of liver injury in humans infected with SARS-CoV-2. Golden Syrian hamsters are an effective model for experimental reproduction of moderate and self-limiting lung disease during SARS-CoV-2 infection. As observed in humans, this experimental model reproduces lesions of bronchointerstitial pneumonia and pulmonary vascular lesions, including endotheliitis (attachment of lymphoid cells to the luminal surface of endothelium). Extrapulmonary vascular lesions are well documented in COVID-19, but such extrapulmonary vascular lesions have not yet been described in the Golden Syrian hamster model of SARS-CoV-2 infection. The study aimed to evaluate microscopic liver lesions in Golden Syrian hamsters experimentally infected with SARS-CoV-2. In total, 38 conventional Golden Syrian hamsters, divided into infected group (n=24) and mock-infected group (n=14), were euthanized at 2-, 3-, 4-, 5-, 7-, 14-, and 15-days post infection with SARS-CoV-2. Liver fragments were evaluated by histopathology and immunohistochemical detection of SARS-CoV-2 Spike S2 antigens. The frequencies of portal vein endotheliitis, lobular activity, hepatocellular degeneration, and lobular vascular changes were higher among SARS-CoV-2-infected animals. Spike S2 antigen was not detected in liver. The main results indicate that SARS-CoV-2 infection exacerbated vascular and inflammatory lesions in the liver of hamsters with pre-existing hepatitis of unknown origin. A potential application of this animal model in studies of the pathogenesis and evolution of liver lesions associated with SARS-CoV-2 infection still needs further evaluation.
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页数:6
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