Identification and characterization of autoallododecaploid barnyard grass (Echinochloa crus-galli L.) induced by in vitro colchicine treatment

被引：0

作者：

Liu, Keyi ^{[1
]}

Zhou, Xiaoling ^{[2
]}

Wang, Wei ^{[1
,3
]}

Wu, Xiaoxiao ^{[1
]}

Zhao, Ziwei ^{[1
]}

Yang, Xiaolu ^{[1
]}

Feng, Ziyi ^{[1
]}

Lv, Pincang ^{[1
]}

Wang, Jie ^{[1
]}

Zhu, Wangwen ^{[1
]}

Zhang, Xianhua ^{[1
]}

He, Yuchi ^{[1
]}

Cai, Detian ^{[1
,3
]}

Song, Zhaojian ^{[1
]}

机构：

[1] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China

[2] Agr Anim Husb & Sci & Technol Bur Siziwang Banner, Ulanqab 011800, Peoples R China

[3] Wuhan Polyploid Biotechnol Co Ltd, Wuhan 430345, Peoples R China

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2024年 / 60卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Autoallododecaploid; Chromosome doubling; Colchicine; Echinochloa crus-galli L; Flow cytometry; Phenotypic variation; WEED MANAGEMENT; POLYPLOIDY; AUTOTETRAPLOIDS; DOMESTICATION; INDUCTION; POACEAE; CULTURE; GROWTH; PLANTS; SHOWS;

D O I：

10.1007/s11627-024-10461-w

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A protocol for in vitro induction of autoallododecaploid Echinochloa crus-galli with colchicine treatment was established. Colchicine concentration and exposure time had significant effects on the callus survival rate, shoot differentiation rate, and chromosome doubling rate. With the increase of colchicine concentration and the extension of treatment time, the callus survival rate and shoot differentiation rate both decreased. Treatment with 0.05% colchicine for 48 hr was the most effective condition for polyploidization, yielding 42.99% dodecaploids. The authenticity of the induced dodecaploid was confirmed by nuclear DNA content, chromosome number, and stomatal and morphological characteristics. Dodecaploid plants showed the obvious "giant" effects of polyploid organs, as well as significantly reduced seed set rate. The concentrations of calcium, iron, most free amino acids, and total free amino acids in dodecaploid plants were significantly higher than those in hexaploid plants. The results showed that the chromosome of allohexaploid E. crus-galli could be redoubled. The dodecaploid E. crus-galli could still grow normally and showed advantages associated with polyploidization, such as enlarged organs and increased nutrient concentrations. The study enriched the germplasm resources of barnyard grass and laid a foundation for the utilization and theoretical research of E. crus-galli in the future.

引用

页码：817 / 828

页数：12

共 52 条

[1] Genetic relationship between Echinochloa crus-galli and Echinochloa oryzicola accessions inferred from internal transcribed spacer and chloroplast DNA sequences
Aoki, Daisuke
Yamaguchi, Hirofumi
[J]. WEED BIOLOGY AND MANAGEMENT, 2008, 8 (04) : 233 - 242
[2] Roles of glycine betaine and proline in improving plant abiotic stress resistance
Ashraf, M.
Foolad, M. R.
[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2007, 59 (02) : 206 - 216
[3] Genome size is a strong predictor of cell size and stomatal density in angiosperms
Beaulieu, Jeremy M.
Leitch, Ilia J.
Patel, Sunil
Pendharkar, Arjun
Knight, Charles A.
[J]. NEW PHYTOLOGIST, 2008, 179 (04) : 975 - 986
[4] Biswas SC., 2001, BANGLADESH J PLANT B, V14, P9
[5] Methods of inducing doubling of chromosomes in plants - By treatment with colchicine
Blakeslee, AF
Avery, AG
[J]. JOURNAL OF HEREDITY, 1937, 28 (12) : 393 - +
[6] Ecological studies on Echinochloa crus-galli and the implications for weed management in direct-seeded rice
Chauhan, B. S.
Johnson, D. E.
[J]. CROP PROTECTION, 2011, 30 (11) : 1385 - 1391
[7] Chen Neng Chen Neng, 2006, Acta Agronomica Sinica, V32, P1193
[8] Genetic and epigenetic mechanisms for gene expression and phenotypic variation in plant polyploids
Chen, Z. Jeffrey
[J]. ANNUAL REVIEW OF PLANT BIOLOGY, 2007, 58 : 377 - 406
[9] CHU CC, 1975, SCI SINICA, V18, P659
[10] The advantages and disadvantages of being polyploid
Comai, L
[J]. NATURE REVIEWS GENETICS, 2005, 6 (11) : 836 - 846

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