Preparation and characterization of PVA/chitosan nanofibers loaded with Dragon's blood or poly helixan as wound dressings

被引：0

作者：

Kancha, Maral Mahboubi ^{[1
,2
]}

Mehrabi, Mohsen ^{[2
]}

Aghaie, Faeze ^{[1
,3
]}

Bitaraf, Fatemeh Sadat ^{[3
]}

Dehghani, Farzaneh ^{[4
]}

Bernkop-Schnuerch, Andreas ^{[5
]}

机构：

[1] Shahroud Univ Med Sci, Student Res Comm, Sch Med, Shahroud, Iran

[2] Shahroud Univ Med Sci, Sch Med, Dept Med Nanotechnol, Shahroud, Iran

[3] Shahroud Univ Med Sci, Sch Med, Dept Med Biotechnol, Shahroud, Iran

[4] Sharif Univ Technol, Dept Chem & Petr Engn, Tehran, Iran

[5] Univ Innsbruck, Inst Pharm, Ctr Chem & Biomed, Dept Pharmaceut Technol, A-6020 Innsbruck, Austria

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 272卷

关键词：

Nanofiber; Wound dressing; Chitosan; FIBROBLAST-GROWTH-FACTOR; SUSTAINED DRUG-RELEASE; ELECTROSPUN NANOFIBERS; STEM-CELLS; IN-VITRO; MECHANICAL-PROPERTIES; ALGINATE HYDROGEL; BARRIER FUNCTION; CHITOSAN; SKIN;

D O I：

10.1016/j.ijbiomac.2024.132844

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanofibers have been investigated in regenerative medicine. Dragon's blood (DB)- and poly helixan PF (PHPF) are natural materials used in cosmetics. Herein, we generated DB- and PHPF-loaded polyvinyl alcohol/chitosan (PVA/CS/DB and PVA/CS/PHPF, respectively) nanofibers. PVA/CS/DB and PVA/CS/PHPF nanofibers had an average diameter of 547.5 +/- 17.13 and 521 +/- 24.67 nm, respectively as assessed by SEM, and a degradation rate of 43.1 and 47.6 % after 14 days, respectively. PVA/CS/DB and PVA/CS/PHPF nanofibers had a hemolysis rate of 0.10 and 0.39 %, respectively, and a water vapor transmission rate of similar to 2200 g.m(-2).day(- 1). These nanofibers exhibited favorable antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis in vitro. PVA/CS/DB and PVA/CS/PHPF nanofibers demonstrated a sustained release of 77.91 and 76.55 % over 72 h. PVA/CS/DB and PVA/CS/PHPF nanofibers had a high rate of cytocompatibility and significantly improved the viability of NIH/3T3 cells as compared with free drugs or unloaded nanofibers. Histological inspection via H&E and Verhoeff's staining demonstrated PVA/CS/DB and PVA/CS/PHPF nanofibers enhanced the wound healing and damaged tissue recovery of unsplinted wound models by promoting epithelial layer formation, collagen deposition, and enhancing the presence of fibroblasts. Conclusively, PVA/ CS/DB and PVA/CS/PHPF can be introduced as potential wound dressing candidates with favorable properties.

引用

页数：16