Biomimetic Proteoglycans Strengthen the Pericellular Matrix of Normal and Osteoarthritic Human Cartilage

被引：3

作者：

Kahle, Elizabeth R. ^{[1
]}

Fallahi, Hooman ^{[1
]}

Bergstrom, Annika R. ^{[2
]}

Li, Anita ^{[3
]}

Trouillot, Colette E. ^{[1
]}

Mulcahey, Mary K. ^{[4
]}

Lu, X. Lucas ^{[5
]}

Han, Lin ^{[1
]}

Marcolongo, Michele S. ^{[3
]}

机构：

[1] Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USA

[2] Villanova Univ, Dept Chem & Biol Engn, Villanova, PA 19085 USA

[3] Villanova Univ, Dept Mech Engn, Villanova, PA 19085 USA

[4] Loyola Univ, Dept Orthopaed Surg & Rehabil, Med Ctr, Maywood, IL 60153 USA

[5] Univ Delaware, Dept Mech Engn, Newark, DE 19716 USA

来源：

ACS BIOMATERIALS SCIENCE & ENGINEERING | 2024年 / 10卷 / 09期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

biomimetic proteoglycan; pericellular matrix; osteoarthritis; IF-guided AFM; molecular engineering; VI COLLAGEN; DECORIN; HISTOPATHOLOGY; NETWORK;

D O I：

10.1021/acsbiomaterials.4c00813

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

In osteoarthritis (OA), degradation of cartilage pericellular matrix (PCM), the proteoglycan-rich immediate cell microniche, is a leading event of disease initiation. This study demonstrated that biomimetic proteoglycans (BPGs) can diffuse into human cartilage from both normal and osteoarthritic donors and are preferentially localized within the PCM. Applying immunofluorescence (IF)-guided AFM nanomechanical mapping, we show that this localization of BPGs increases the PCM micromodulus of both normal and OA specimens. These results illustrate the capability of BPGs to integrate with degenerative tissues and support the translational potential of BPGs for treating human OA and other diseases associated with proteoglycan degradation.

引用

页码：5617 / 5623

页数：7

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