Quantitation of the main constituents of vanilla by reverse phase HPLC and ultra-high-pressure-liquid-chromatography with UV detection: Method validation and performance comparison

Vanilla's main constituents, i.e., vanillin, para-hydroxybenzaldehyde, and their corresponding acids, can be easily quantified by RP LC with UV detection and external calibration. This paper describes two methods that were developed using HPLC and ultra-high-pressure LC (UHPLC), respectively, and validated according to the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) [1]. Both methods were highly specific, exhibited good linearities with high precision, and achieved good accuracies of quantitative results. The UHPLC method was more sensitive, five times shorter, and gave better peak resolutions than the HPLC alternative.