Nanocomposite Based on Bacterial Cellulose and Silver Nanoparticles Improve Wound Healing Without Exhibiting Toxic Effect

被引：7

作者：

Ozelin, Saulo Duarte ^{[1
]}

Esperandima, Tabata Rodrigues ^{[1
]}

Dias, Fernanda Gosuen Goncalves ^{[1
]}

Pereira, Lucas de Freitas ^{[1
]}

Garcia, Cristiane Buzatto ^{[1
]}

de Souza, Thiago Olimpio ^{[1
]}

Magalhaes, Larissa Fernandes ^{[1
]}

Barud, Hernane da Silva ^{[2
,3
]}

Sabio, Rafael Miguel ^{[4
]}

Tavares, Denise Crispim ^{[1
]}

机构：

[1] Univ Franca, Ave Dr Armando Salles de Oliveira,201 Parque Univ, BR-14404600 Franca, SP, Brazil

[2] Univ Araraquara, Biopolymers & Biomat Lab, Rua Carlos Comes 1338, BR-14801320 Araraquara, SP, Brazil

[3] BioSmart Nano, Ave Jorge Fernandes de Mattos 311, BR-14808162 Araraquara, SP, Brazil

[4] Sao Paulo State Univ, Sch Pharmaceut Sci, Rodovia Araraquara Jau, Km 01, BR-14800903 Araraquara, SP, Brazil

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 2024年 / 113卷 / 08期

基金：

巴西圣保罗研究基金会;

关键词：

Bacterial cellulose; Silver nanoparticles; Wound healing assay; Antimicrobial activity; Toxicological assay; BIOFILMS; SKIN;

D O I：

10.1016/j.xphs.2024.04.010

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Wound healing is an important and complex process, containing a multifaceted process governed by sequential yet overlapping phases. Certain treatments can optimize local physiological conditions and improve wound healing. Silver nanoparticles (AgNP) are widely known for their antimicrobial activity. On the other hand, bacterial cellulose (BC) films have been used as a dressing that temporarily substitutes the skin, offering many advantages in optimizing wound healing, in addition to being highly biocompatible. Considering the promising activities of AgNP and BC films, the present study aimed to evaluate the wound healing activity in Wistar Hannover rats using a nanocomposite based on bacterial cellulose containing AgNP (AgBC). In a period of 21 days, its influence on the wound area, microbial growth, histopathological parameters, and collagen content were analyzed. In addition, toxicity indicators were assessed, such as weight gain, water consumption, and creatinine and alanine transaminase levels. After 14 days of injury, the animals treated with AgBC showed a significant increase in wound contraction. The treatment with AgBC significantly reduced the number of microbial colonies compared to other treatments in the first 48 h after the injury. At the end of the 21 experimental days, an average wound contraction rate greater than 97 % in relation to the initial area was observed, in addition to a significant increase in the amount of collagen fibers at the edge of the wounds, lower scores of necrosis, angiogenesis and inflammation, associated with no systemic toxicity. Therefore, it is concluded that the combination of preexisting products to form a new nanocomposite based on BC and AgNP amplified the biological activity of these products, increasing the effectiveness of wound healing and minimizing possible toxic effects of silver. (c) 2024 American Pharmacists Association. Published by Elsevier Inc. All rights reserved.

引用

页码：2383 / 2393

页数：11

共 82 条

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