A cytidine deaminase regulates axon regeneration by modulating the functions of the Caenorhabditis elegans HGF/plasminogen family protein SVH-1

被引：1

作者：

Shimizu, Tatsuhiro ^{[1
]}

Nomachi, Takafumi ^{[1
]}

Matsumoto, Kunihiro ^{[1
]}

Hisamoto, Naoki ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Nagoya, Japan

来源：

PLOS GENETICS | 2024年 / 20卷 / 07期

基金：

日本学术振兴会;

关键词：

B MESSENGER-RNA; APOLIPOPROTEIN-B; SECONDARY STRUCTURE; C; ELEGANS; KINESIN-1; BINDING; SITE;

D O I：

10.1371/journal.pgen.1011367

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The pathway for axon regeneration in Caenorhabditis elegans is activated by SVH-1, a growth factor belonging to the HGF/plasminogen family. SVH-1 is a dual-function factor that acts as an HGF-like growth factor to promote axon regeneration and as a protease to regulate early development. It is important to understand how SVH-1 is converted from a protease to a growth factor for axon regeneration. In this study, we demonstrate that cytidine deaminase (CDD) SVH-17/CDD-2 plays a role in the functional conversion of SVH-1. We find that the codon exchange of His-755 to Tyr in the Asp-His-Ser catalytic triad of SVH-1 can suppress the cdd-2 defect in axon regeneration. Furthermore, the stem hairpin structure around the His-755 site in svh-1 mRNA is required for the activation of axon regeneration by SVH-1. These results suggest that CDD-2 promotes axon regeneration by transforming the function of SVH-1 from a protease to a growth factor through modification of svh-1 mRNA.

引用

页数：15

共 32 条

[1] An AU-rich sequence element (UUUN[A/U]U) downstream of the edited C in apolipoprotein B mRNA is a high-affinity binding site for Apobec-1:: Binding of Apobec-1 to this motif in the 3′ untranslated region of c-myc increases mRNA stability [J].

Anant, S ;

Davidson, NO .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (06) :1982-1992

[2]

Andrew JM., 1995, J Biol Chem, V270, P14768

[3] The Caenorhabditis elegans JIP3 Protein UNC-16 Functions As an Adaptor to Link Kinesin-1 with Cytoplasmic Dynein [J].

Arimoto, Makoto ;

Koushika, Sandhya P. ;

Choudhary, Bikash C. ;

Li, Chris ;

Matsumoto, Kunihiro ;

Hisamoto, Naoki .

JOURNAL OF NEUROSCIENCE, 2011, 31 (06) :2216-2224

[4] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[5] APOBEC-1-mediated RNA editing [J].

Blanc, Valerie ;

Davidson, Nicholas O. .

WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE, 2010, 2 (05) :594-602

[6]

BRENNER S, 1974, GENETICS, V77, P71

[7] Axon Regeneration Pathways Identified by Systematic Genetic Screening in C. elegans [J].

Chen, Lizhen ;

Wang, Zhiping ;

Ghosh-Roy, Anindya ;

Hubert, Thomas ;

Yan, Dong ;

O'Rourke, Sean ;

Bowerman, Bruce ;

Wu, Zilu ;

Jin, Yishi ;

Chisholm, Andrew D. .

NEURON, 2011, 71 (06) :1043-1057

[8] Apolipoprotein B: mRNA editing, lipoprotein assembly, and presecretory degradation [J].

Davidson, NO ;

Shelness, GS .

ANNUAL REVIEW OF NUTRITION, 2000, 20 :169-+

[9] Beyond Trophic Factors: Exploiting the Intrinsic Regenerative Properties of Adult Neurons [J].

Duraikannu, Arul ;

Krishnan, Anand ;

Chandrasekhar, Ambika ;

Zochodne, Douglas W. .

FRONTIERS IN CELLULAR NEUROSCIENCE, 2019, 13

[10] Heritable genome editing in C. elegans via a CRISPR-Cas9 system [J].

Friedland, Ari E. ;

Tzur, Yonatan B. ;

Esvelt, Kevin M. ;

Colaiacovo, Monica P. ;

Church, George M. ;

Calarco, John A. .

NATURE METHODS, 2013, 10 (08) :741-+

← 1 2 3 4 →