Aspergillus fumigatus conidial surface-associated proteome reveals factors for fungal evasion and host immunity modulation

被引：1

作者：

Pinzan, Camila Figueiredo ^{[1
]}

Valero, Clara ^{[1
,2
]}

de Castro, Patricia Alves ^{[1
]}

da Silva, Jefferson Luiz ^{[1
]}

Earle, Kayleigh ^{[2
]}

Liu, Hong ^{[3
]}

Horta, Maria Augusta Crivelente ^{[1
]}

Kniemeyer, Olaf ^{[4
,5
]}

Kruger, Thomas ^{[4
,5
]}

Pschibul, Annica ^{[4
,5
]}

Comert, Derya Nur ^{[4
,5
]}

Heinekamp, Thorsten ^{[4
,5
]}

Brakhage, Axel A. ^{[4
,5
]}

Steenwyk, Jacob L. ^{[6
,7
]}

Mead, Matthew E. ^{[8
]}

Hermsdorf, Nico ^{[4
,5
]}

Filler, Scott G. ^{[3
,9
]}

da Rosa-Garzon, Nathalia Gonsales ^{[1
]}

Delbaje, Endrews ^{[1
]}

Bromley, Michael J. ^{[2
,10
]}

Cabral, Hamilton ^{[1
]}

Diehl, Camila ^{[1
]}

Angeli, Claudia B. ^{[11
]}

Palmisano, Giuseppe ^{[11
]}

Ibrahim, Ashraf S. ^{[3
,9
]}

Rinker, David C. ^{[8
]}

Sauters, Thomas J. C. ^{[8
]}

Steffen, Karin ^{[8
]}

Gumilang, Adiyantara ^{[8
]}

Rokas, Antonis ^{[8
]}

Gago, Sara ^{[2
]}

dos Reis, Thaila F. ^{[1
]}

Goldman, Gustavo H. ^{[1
,12
]}

机构：

[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, Brazil

[2] Univ Manchester, Fac Biol Med & Hlth, Div Evolut Infect & Genom, Manchester Fungal Infect Grp, Manchester, England

[3] UCLA, Lundquist Inst Biomed Innovat Harbor, Med Ctr, Div Infect Dis, Torrance, CA USA

[4] Friedrich Schiller Univ, Leibniz Inst Nat Prod Res & Infect Biol Leibniz HK, Dept Mol & Appl Microbiol, Jena, Germany

[5] Friedrich Schiller Univ, Inst Microbiol, Jena, Germany

[6] Univ Calif Berkeley, Howards Hughes Med Inst, Berkeley, CA USA

[7] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA USA

[8] Vanderbilt Univ, Dept Biol Sci & Evolutionary Studies Initiat, Nashville, TN 37235 USA

[9] UCLA, David Geffen Sch Med, Los Angeles, CA USA

[10] Univ Manchester, Antimicrobial Resistance Network, Manchester, England

[11] Univ Sao Paulo, Dept Parasitol, Inst Ciencias Biomed, Sao Paulo, Brazil

[12] Natl Inst Sci & Technol Human Pathogen Fungi, Sao Paulo, Brazil

来源：

NATURE MICROBIOLOGY | 2024年 / 9卷 / 10期

基金：

以色列科学基金会; 巴西圣保罗研究基金会; 英国医学研究理事会; 英国惠康基金; 英国国家替代、减少和改良动物研究中心; 加拿大健康研究院; 美国国家卫生研究院;

关键词：

PROTEINS; CELLS;

D O I：

10.1038/s41564-024-01782-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aspergillus fumigatus causes aspergillosis and relies on asexual spores (conidia) for initiating host infection. There is scarce information about A. fumigatus proteins involved in fungal evasion and host immunity modulation. Here we analysed the conidial surface proteome of A. fumigatus, two closely related non-pathogenic species, Aspergillus fischeri and Aspergillus oerlinghausenensis, as well as pathogenic Aspergillus lentulus, to identify such proteins. After identifying 62 proteins exclusively detected on the A. fumigatus conidial surface, we assessed null mutants for 42 genes encoding these proteins. Deletion of 33 of these genes altered susceptibility to macrophage, epithelial cells and cytokine production. Notably, a gene that encodes a putative glycosylasparaginase, modulating levels of the host proinflammatory cytokine IL-1 beta, is important for infection in an immunocompetent murine model of fungal disease. These results suggest that A. fumigatus conidial surface proteins are important for evasion and modulation of the immune response at the onset of fungal infection.

引用

页码：2710 / 2726

页数：30