Genome-Wide Analysis of the WOX Family and Its Expression Pattern in Root Development of Paeonia ostii

被引:0
作者
Lou, Xueyuan [1 ]
Wang, Jiange [2 ]
Wang, Guiqing [2 ]
He, Dan [2 ]
Shang, Wenqian [2 ]
Song, Yinglong [2 ]
Wang, Zheng [2 ]
He, Songlin [2 ]
机构
[1] Henan Agr Univ, Coll Hort, Zhengzhou 450046, Peoples R China
[2] Henan Agr Univ, Coll Landscape Architecture & Art, Zhengzhou 450002, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Paeonia ostii; WOX family; root development; bioinformatics; expression analysis; STEM-CELL MAINTENANCE; HOMEOBOX GENE; TRANSCRIPTION FACTOR; HOMEODOMAIN PROTEIN; RECEPTOR KINASE; ARABIDOPSIS; WUSCHEL; SHOOT; FATE; RICE;
D O I
10.3390/ijms25147668
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tree peony (Paeonia suffruticosa Andr.) is a woody plant with high ornamental, medicinal, and oil values. However, its low rooting rate and poor rooting quality are bottleneck issues in the micropropagation of P. ostii. The WUSCHEL-related homeobox (WOX) family plays a crucial role in root development. In this study, based on the screening of the genome and root transcriptome database, we identified ten WOX members in P. ostii. Phylogenetic analysis revealed that the ten PoWOX proteins clustered into three major clades, the WUS, intermediate, and ancient clade, respectively. The conserved motifs and tertiary structures of PoWOX proteins located in the same clade exhibited higher similarity. The analysis of cis-regulatory elements in the promoter indicated that PoWOX genes are involved in plant growth and development, phytohormones, and stress responses. The expression analysis revealed that PoWOX genes are expressed in distinct tissues. PoWOX4, PoWOX5, PoWOX11, and PoWOX13b are preferentially expressed in roots at the early stage of root primordium formation, suggesting their role in the initiation and development of roots. These results will provide a comprehensive reference for the evolution and potential function of the WOX family and offer guidance for further study on the root development of tree peony.
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页数:19
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