CHCHD4 regulates the expression of mitochondrial genes that are essential for tumour cell growth

被引：0
|
作者：
Thomas, Luke W. [1 ]
Stephen, Jenna M. [1 ]
Ashcroft, Margaret [1 ]
机构：
[1] Univ Cambridge, Dept Med, Cambridge Biomed Campus, Cambridge CB2 0QQ, England
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 2024年 / 1870卷 / 07期
基金：
英国医学研究理事会; 英国惠康基金;
关键词：
Mitochondria; CHCHD4; SILAC analysis; Proteomics; CRISPR/Cas9 genome-wide deletion screen; Gene essentiality;
D O I：
10.1016/j.bbadis.2024.167282
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
CHCHD4 (MIA40) is central to the functions of the mitochondrial disulfide relay system (DRS). CHCHD4 is essential and evolutionarily conserved. Previously, we have shown CHCHD4 to be a critical regulator of tumour cell growth. Here, we use integrated analysis of our genome-wide CRISPR/Cas9 and SILAC proteomic screening data to delineate mechanisms of CHCHD4 essentiality in cancer. We identify a shortlist of common essential genes/proteins regulated by CHCHD4, including subunits of complex I that are known DRS substrates, and genes/proteins involved in key metabolic pathways. Our study highlights a range of CHCHD4-regulated nuclear encoded mitochondrial genes/proteins essential for tumour cell growth.
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页数：4
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