Intraocular fatty acids induce reinforcement of barrier functions on the outer blood-retinal barrier

被引:1
作者
Nishikiori, Nami [1 ]
Watanabe, Megumi [1 ]
Sato, Tatsuya [2 ,3 ]
Umetsu, Araya [1 ]
Higashide, Megumi [1 ]
Furuhashi, Masato [2 ]
Ohguro, Hiroshi [1 ]
机构
[1] Sapporo Med Univ, Sch Med, Dept Ophthalmol, Sapporo, Japan
[2] Sapporo Med Univ, Sch Med, Dept Cardiovasc Renal & Metab Med, Sapporo, Japan
[3] Sapporo Med Univ, Sch Med, Dept Cellular Physiol & Signal Transduct, Sapporo, Japan
来源
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 2024年 / 202卷
关键词
Fatty acids; Extracellular flux analysis; Retinal pigment epithelium; Outer blood-retinal barrier; DOCOSAHEXAENOIC ACID; PIGMENT EPITHELIUM; MACULAR DEGENERATION; PHOSPHOLIPIDS; DEFICIENCY; METABOLISM; JUNCTIONS;
D O I
10.1016/j.plefa.2024.102637
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The aim of the present study was to elucidate unknown effects of intraocular fatty acids (ioFAs) including palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), arachidonic acid (C20:4), eicosapentaenoic acid (EPA, C20:5) and docosahexaenoic acid (DHA, C22:6) on the outer blood-retinal barrier (oBRB). For this purpose, human retinal pigment epithelium cell line ARPE19 was subjected to analyses for evaluating the following biological phenotypes: (1) cell viability, (2) cellular metabolic functions, (3) barrier functions by trans-epithelial electrical resistance (TEER), and (4) expression of tight junction (TJ) molecules. In the presence of 100 nM ioFAs, no significant effects on cell viability of ARPE19 cells was observed. While treatment with EPA or DHA tended to reduce non-mitochondrial oxygen consumption, most indices in mitochondrial functions were not markedly affected by treatment with ioFAs in ARPE19 cells. On the other hand, ioFAs except for palmitic acid and stearic acid significantly increased basal extracellular acidification rates, suggesting activated glycolysis or increased lactate production. Interestingly, TEER values of planar ARPE19 monolayer were significantly increased by treatment any ioFAs. Consistently, gene expression levels of TJ proteins were increased by treatment with ioFAs. Collectively, the findings presented herein suggest that ioFAs may contribute to reinforcement of barrier functions of the oBRB albeit there are some differences in biological effects depending on the type of ioFAs.
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相关论文
共 43 条
[1]   Human Retinal Pigment Epithelium Cells as Functional Models for the RPE In Vivo [J].
Ablonczy, Zsolt ;
Dahrouj, Mohammad ;
Tang, Peter H. ;
Liu, Yueying ;
Sambamurti, Kumar ;
Marmorstein, Alan D. ;
Crosson, Craig E. .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2011, 52 (12) :8614-8620
[2]   Lipid Composition of the Human Eye: Are Red Blood Cells a Good Mirror of Retinal and Optic Nerve Fatty Acids? [J].
Acar, Niyazi ;
Berdeaux, Olivier ;
Gregoire, Stephane ;
Cabaret, Stephanie ;
Martine, Lucy ;
Gain, Philippe ;
Thuret, Gilles ;
Creuzot-Garcher, Catherine P. ;
Bron, Alain M. ;
Bretillon, Lionel .
PLOS ONE, 2012, 7 (04)
[3]   The dynamic nature of Bruch's membrane [J].
Booij, J. C. ;
Baas, D. C. ;
Beisekeeva, J. ;
Gorgels, T. G. M. F. ;
Bergen, A. A. B. .
PROGRESS IN RETINAL AND EYE RESEARCH, 2010, 29 (01) :1-18
[4]   Lipid and fatty acid profile of the retina, retinal pigment epithelium/choroid, and the lacrimal gland, and associations with adipose tissue fatty acids in human subjects [J].
Bretillon, Lionel ;
Thuret, Gilles ;
Gregoire, Stephane ;
Acar, Niyazi ;
Joffre, Corinne ;
Bron, Alain M. ;
Gain, Philippe ;
Creuzot-Garcher, Catherine P. .
EXPERIMENTAL EYE RESEARCH, 2008, 87 (06) :521-528
[5]   Blood-retinal barrier [J].
Cunha-Vaz, Jose ;
Bernardes, Rui ;
Lobo, Conceicao .
EUROPEAN JOURNAL OF OPHTHALMOLOGY, 2011, 21 :S3-S9
[6]   VERTEBRATE ROD OUTER SEGMENT MEMBRANES [J].
DAEMEN, FJM .
BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 300 (03) :255-288
[7]  
FLIESLER SJ, 1983, PROG LIPID RES, V22, P79
[8]   Lipid metabolism and retinal diseases [J].
Gabrielle, Pierre-Henry .
ACTA OPHTHALMOLOGICA, 2022, 100 :3-43
[9]   Cytoarchitecture of choroidal capillary endothelial cells [J].
Guymer, RH ;
Bird, AC ;
Hageman, GS .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 (06) :1660-1666
[10]   Triglyceride-derived fatty acids reduce autophagy in a model of retinal angiomatous proliferation [J].
Heckel, Emilie ;
Cagnone, Gael ;
Agnihotri, Tapan ;
Cakir, Bertan ;
Das, Ashim ;
Kim, Jin Sung ;
Kim, Nicholas ;
Lavoie, Genevieve ;
Situ, Anu ;
Pundir, Sheetal ;
Sun, Ye ;
Wunnemann, Florian ;
Pierce, Kerry A. ;
Dennis, Courtney ;
Mitchell, Grant A. ;
Chemtob, Sylvain ;
Rezende, Flavio A. ;
Andelfinger, Gregor ;
Clish, Clary B. ;
Roux, Philippe P. ;
Sapieha, Przemyslaw ;
Smith, Lois Eh ;
Joyal, Jean-Sebastien .
JCI INSIGHT, 2022, 7 (06)