Advancements of CRISPR-Mediated Base Editing in Crops and Potential Applications in Populus

被引：1
作者：
Yang, Xuefei [1 ]
Zhu, Ping [1 ]
Gui, Jinshan [1 ]
机构：
[1] Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 15期
基金：
中国国家自然科学基金;
关键词：
CRISPR-Cas9; genome editing; base editor; crop; Populus; GENOMIC DNA; TARGET BASE; RICE; RESISTANCE; EVOLUTION; REPEATS; EDITORS; REPAIR; SCOPE;
D O I：
10.3390/ijms25158314
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Base editing represents a cutting-edge genome editing technique that utilizes the CRISPR system to guide base deaminases with high precision to specific genomic sites, facilitating the targeted alteration of individual nucleotides. Unlike traditional gene editing approaches, base editing does not require DNA double-strand breaks or donor templates. It functions independently of the cellular DNA repair machinery, offering significant advantages in terms of both efficiency and accuracy. In this review, we summarize the core design principles of various DNA base editors, their distinctive editing characteristics, and tactics to refine their efficacy. We also summarize their applications in crop genetic improvement and explore their potential contributions to forest genetic engineering.
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页数：14
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