Purification and Characterization of α-Mannosidase from Onion, Allium cepa

被引：0
|
作者：
Narita, Yui [1 ]
Tatara, Yota [2 ]
Hamada, Shigeki [1 ]
Kojima, Kaoru [1 ]
Li, Shuai [1 ]
Yoshida, Takashi [1 ]
机构：
[1] Hirosaki Univ, Dept Biochem & Mol Biol, Fac Agr & Life Sci, 3 Bunkyo, Hirosaki, Aomori 0368561, Japan
[2] Hirosaki Univ, Dept Stress Response Sci, Ctr Adv Med Res, Grad Sch Med, 5 Zaifu Cho, Hirosaki, Aomori 0368562, Japan
来源：
JOURNAL OF APPLIED GLYCOSCIENCE | 2024年 / 71卷 / 01期
关键词：
alpha-Mannosidase; onion; Allium cepa; PENICILLIUM-CITRINUM; N-GLYCANS; TURNOVER; GENOME;
D O I：
10.5458/jag.jag.JAG-2023_0010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
alpha-Mannosidase (ALMAN) extracted from onion (Allium cepa) was purified by column chromatography such as hydrophobic and gel filtration. ALMAN is an acidic alpha-mannosidase that exhibits maximum activity against pNP-alpha-Man at pH 4.0-5.0 at 50 degrees C. Amino acid sequence analysis of ALMAN was consistent with alpha-mannosidase deduced from Allium cepa transcriptome analysis. The gene alman was amplified by PCR using mRNA extracted from onions, and a full-length gene of 3,054 bp encoding a protein of 1,018 amino acid residues was revealed. ALMAN is classified as Glycoside Hydrolase Family (GH) 38 and showed homology with other plant-derived alpha-mannosidases such as tomato and hot pepper.
引用
收藏
页码：33 / 36
页数：4
相关论文
共 50 条
  • [1] Purification and cloning of a γ-glutamyl transpeptidase from onion (Allium cepa)
    Shaw, ML
    Pither-Joyce, MD
    McCallum, JA
    PHYTOCHEMISTRY, 2005, 66 (05) : 515 - 522
  • [2] CHARACTERIZATION OF ALLIINASE OF ALLIUM CEPA (ONION)
    SCHWIMMER, S
    MAZELIS, M
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1963, 100 (01) : 66 - +
  • [3] Immunological characterization of onion (Allium cepa) allergy
    Albanesi, Marcello
    Pasculli, Carlo
    Giliberti, Lucia
    Rossi, Maria Pia
    Di Bona, Danilo
    Caiaffa, Maria Filomena
    Macchia, Luigi
    POSTEPY DERMATOLOGII I ALERGOLOGII, 2019, 36 (01): : 98 - 103
  • [4] Flavonoids from red onion (Allium cepa)
    Fossen, T
    Pedersen, AT
    Andersen, OM
    PHYTOCHEMISTRY, 1998, 47 (02) : 281 - 285
  • [5] Allium Cepa: The Humble Onion
    Longo, Perie
    STAND, 2012, 10 (04): : 76 - 76
  • [6] Purification and characterization of crude fructooligosaccharides extracted from red onion (Allium cepa var. viviparum) by yeast treatment
    Aisara, Jakkrit
    Wongsanittayarak, Jirat
    Leangnim, Nalapat
    Utama, Kraikrit
    Sangthong, Padchanee
    Sriyotai, Woraprapa
    Mahatheeranont, Sugunya
    Phongthai, Suphat
    Unban, Kridsada
    Lumyong, Saisamorn
    Khanongnuch, Chartchai
    Wongputtisin, Pairote
    Kanpiengjai, Apinun
    MICROBIAL CELL FACTORIES, 2024, 23 (01)
  • [7] Purification and characterization of crude fructooligosaccharides extracted from red onion (Allium cepa var. viviparum) by yeast treatment
    Jakkrit Aisara
    Jirat Wongsanittayarak
    Nalapat Leangnim
    Kraikrit Utama
    Padchanee Sangthong
    Woraprapa Sriyotai
    Sugunya Mahatheeranont
    Suphat Phongthai
    Kridsada Unban
    Saisamorn Lumyong
    Chartchai Khanongnuch
    Pairote Wongputtisin
    Apinun Kanpiengjai
    Microbial Cell Factories, 23
  • [8] ISOLATION AND CHARACTERIZATION OF THE NUCLEAR MATRIX OF THE ONION ALLIUM-CEPA
    STOLYAROV, SD
    TSITOLOGIYA, 1984, 26 (08): : 874 - +
  • [9] A new disease of the onion (Allium cepa)
    Ryzkov, VL
    Vovk, AM
    COMPTES RENDUS DE L ACADEMIE DES SCIENCES DE L URSS, 1937, 16 : 69 - 72
  • [10] LACHRYMATORY FACTOR IN ONION (ALLIUM CEPA)
    KUMAR, SA
    JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH, 1963, 22 (11): : 470 - &
12345