Purification and Characterization of α-Mannosidase from Onion, Allium cepa

被引：0
|
作者：
Narita, Yui [1 ]
Tatara, Yota [2 ]
Hamada, Shigeki [1 ]
Kojima, Kaoru [1 ]
Li, Shuai [1 ]
Yoshida, Takashi [1 ]
机构：
[1] Hirosaki Univ, Dept Biochem & Mol Biol, Fac Agr & Life Sci, 3 Bunkyo, Hirosaki, Aomori 0368561, Japan
[2] Hirosaki Univ, Dept Stress Response Sci, Ctr Adv Med Res, Grad Sch Med, 5 Zaifu Cho, Hirosaki, Aomori 0368562, Japan
来源：
JOURNAL OF APPLIED GLYCOSCIENCE | 2024年 / 71卷 / 01期
关键词：
alpha-Mannosidase; onion; Allium cepa; PENICILLIUM-CITRINUM; N-GLYCANS; TURNOVER; GENOME;
D O I：
10.5458/jag.jag.JAG-2023_0010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
alpha-Mannosidase (ALMAN) extracted from onion (Allium cepa) was purified by column chromatography such as hydrophobic and gel filtration. ALMAN is an acidic alpha-mannosidase that exhibits maximum activity against pNP-alpha-Man at pH 4.0-5.0 at 50 degrees C. Amino acid sequence analysis of ALMAN was consistent with alpha-mannosidase deduced from Allium cepa transcriptome analysis. The gene alman was amplified by PCR using mRNA extracted from onions, and a full-length gene of 3,054 bp encoding a protein of 1,018 amino acid residues was revealed. ALMAN is classified as Glycoside Hydrolase Family (GH) 38 and showed homology with other plant-derived alpha-mannosidases such as tomato and hot pepper.
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页码：33 / 36
页数：4
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