Evaluation of muscular apoptotic changes and myogenin gene expression in experimental trichinosis after stem cells and atorvastatin added to ivermectin treatment

被引:0
作者
Hassan, Zeinab R. [1 ,11 ]
El-Sayed, Samar [1 ]
Zekry, Kareman M. [1 ]
Ahmed, Samah G. [2 ]
Abd Elhamid, Asmaa Hassan [2 ]
Salama, Doaa E. A. [3 ,12 ]
Taha, Azza Kamal [3 ]
Mahmoud, Nihal A. [4 ]
Mohammed, Shaymaa Fathy [4 ]
Amin, Mona M. [5 ]
Mohamed, Rasha Elsayed [6 ]
Eraque, Ayat M. S. [6 ]
Mohamed, Shimaa A. [6 ,8 ]
Abdelgalil, Ranya M. [7 ]
Atta, Shimaa Attia
Fahmy, Nermeen Talaat [9 ]
Badr, Mohamed S. [10 ]
机构
[1] Al Azhar Univ, Fac Med Girls, Dept Parasitol, Cairo, Egypt
[2] Al Azhar Univ, Fac Med Girls, Histol, Cairo, Egypt
[3] Al Azhar Univ, Fac Med Girls, Pathol, Cairo, Egypt
[4] Al Azhar Univ, Fac Med Girls, Physiol, Cairo, Egypt
[5] Al Azhar Univ, Fac Med Girls, Pharmacol, Cairo, Egypt
[6] Al Azhar Univ, Fac Med Girls, Biochem, Cairo, Egypt
[7] Al Azhar Univ, Fac Med Girls, Anat, Cairo, Egypt
[8] Theodor Bilharz Res Inst, Immunol, Cairo, Egypt
[9] Egypt Ctr Res & Regenerat Med ECRRM, Mol Biol & Genom, Cairo, Egypt
[10] Ain Shams Univ, Fac Med, Med Res Ctr, Mol Biol & Genet Bioinformat Nanorobot Diagnost, Cairo, Egypt
[11] Benha Natl Univ BNU, Dept Parasitol, Qalyubia, Egypt
[12] Badr Univ Cairo BUC, Fac Med, Dept Pathol, Cairo, Egypt
关键词
Apoptosis; Atorvastatin; Feulgen; Myogenin; Stem cells; Trichinosis; NF-KAPPA-B; TRICHINELLA-SPIRALIS; MICE;
D O I
10.1016/j.exppara.2024.108823
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Trichinosis is a common parasitic disease that affects the striated skeletal muscles, causing apoptotic and degenerative changes associated with myogenin expression in the affected myocytes. Hence, this study aimed to assess the ameliorative effects of stem cells and atorvastatin added to ivermectin on the infected myocytes during the muscular phase of murine trichinosis. 120 laboratory Swiss albino male mice were divided into 10 groups, and each group was subdivided into intestinal and muscular phases (each n = 6); uninfected control; untreated infected control; infected received ivermectin monotherapy; infected received atorvastatin monotherapy; infected received stem cells monotherapy; infected received ivermectin and atorvastatin dual therapy; infected received ivermectin and stem cells dual therapy; infected received atorvastatin and stem cells dual therapy; infected received ivermectin 0.2, atorvastatin 40, and stem cells triple therapy; and infected received ivermectin 0.1, atorvastatin 20, and stem cells triple therapy. Intestinal phase mice were sacrificed on the 5th day postinfection, while those of the muscular phase were sacrificed on the 35th day post-infection. Parasitological, histopathological, ultrastructural, histochemical, biochemical, and myogenin gene expression assessments were performed. The results revealed that mice that received ivermectin, atorvastatin, and stem cell triple therapies showed the maximum reduction in the adult worm and larvae burden, marked improvement in the underlying muscular degenerative changes (as was noticed by histopathological, ultrastructural, and histochemical Feulgen stain assessment), lower biochemical levels of serum NK-kappa B and tissue NO, and lower myogenin expression. Accordingly, the combination of stem cells, atorvastatin, and ivermectin affords a potential synergistic activity against trichinosis with considerable healing of the underlying degenerative sequel.
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页数:11
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