A high-accuracy measurement procedure for salbutamol, ractopamine, and clenbuterol in pork by isotope dilution-liquid chromatography/tandem mass spectrometry

被引:2
作者
Zhou, Xia [1 ,2 ]
Tian, Tian [1 ,3 ]
Li, Xiuqin [1 ,2 ]
Liu, Shuyu [3 ]
Zhang, Qinghe [1 ,2 ]
机构
[1] Natl Inst Metrol, Div Chem Metrol & Analyt Sci, Beijing 100029, Peoples R China
[2] Key Lab Chem Metrol & Applicat Nutr & Hlth State M, Beijing 100029, Peoples R China
[3] Shanghai Univ Engn Sci, Shanghai, Peoples R China
关键词
Enzymatic digestion; Deuterium labelled internal standard; Isotope dilution mass spectrometry; /32-agonists; /32-agonist conjugates; LABELED INTERNAL STANDARDS; BETA-AGONISTS; RESIDUES; LIVER; BETA(2)-AGONISTS; VALIDATION; MUSCLE; URINE; DRUGS; C-13;
D O I
10.1016/j.foodchem.2024.140357
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In-depth research into the precise evaluation of enzymatic digestion efficiency and the selection of a suitable deuterium-labelled internal standard remains a gap in the accurate determination of /32-agonists in animalderived food by isotope dilution-liquid chromatography/tandem mass spectrometry (ID-LC-MS/MS). In this study, the enzymatic digestion conditions were optimized by monitoring the presence of /32-agonist conjugates in positive samples, which proved to be reliable for ensuring complete enzymatic digestion. Comparative analysis of deuterium-labelled internal standards for salbutamol (SAL), ractopamine (RAC), and clenbuterol (CLB) revealed that CLB-D-6 and SAL-D-9 were less effective in compensating for matrix effects due to hydrogen-deuterium exchange during MS fragment formation. Consequently, SAL-D-3, RAC-D-3 and CLB-D-9 were chosen for the implementation of ID-LC-MS/MS. The developed method demonstrates high accuracy and precision, with the average recoveries ranging from 93.8% to 107.3% with RSD <6.1%, which can provide higher-order measurement results for beta(2)-agonists in pork.
引用
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页数:9
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