Evaluation of amlodipine against strains of Candida spp. in planktonic cells, developing biofilms and mature biofilms

被引：0
|
作者：
Queiroz, Helaine Almeida [1 ,2 ]
da Silva, Lisandra Juvencio [1 ,2 ]
Barroso, Fatima Daiana Dias [1 ,2 ,3 ]
Sa, Livia Gurgel do Amaral Valente Valente [1 ,2 ,3 ]
Neto, Joao Batista de Andrade [1 ,2 ,3 ]
da Costa, erica Rayanne Motta [1 ,2 ]
de Oliveira, Leilson Carvalho [1 ,2 ]
Barbosa, Amanda Dias [1 ,2 ]
Cabral, Vitoria Pessoa de Farias [1 ,2 ]
Rodrigues, Daniel Sampaio [1 ,2 ,3 ]
Moreira, Lara Elloyse Almeida [1 ,2 ]
Cavalcanti, Bruno Coelho [2 ,4 ]
Magalhaes, Islay Lima [2 ,4 ]
de Moraes, Manoel Odorico [2 ,4 ]
Nobre Junior, Helio Vitoriano [1 ,2 ]
da Silva, Cecilia Rocha [1 ,2 ]
机构：
[1] Univ Fed Ceara, Sch Pharm, Lab Bioprospect Antimicrobial Mol LABIMAN, Fortaleza, CE, Brazil
[2] Univ Fed Ceara, Drug Res & Dev Ctr, Fortaleza, CE, Brazil
[3] Christus Univ Ctr UNICHRISTUS, Fortaleza, CE, Brazil
[4] Univ Fed Ceara, Dept Physiol & Pharmacol, Fortaleza, CE, Brazil
来源：
FUTURE MICROBIOLOGY | 2024年 / 19卷 / 16期
关键词：
amlodipine; apoptosis; biofilm; Candida spp; fluconazole; FLUCONAZOLE; ALBICANS; RESISTANT;
D O I：
10.1080/17460913.2024.2390286
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: To evaluate the antifungal activity of amlodipine against strains of Candida spp. and to its possible mechanism of action. Methods: Broth microdilution tests were used to determine the minimum inhibitory concentration, while the synergistic activity was evaluated by calculating the fractional inhibitory concentration index. The action of amlodipine against biofilms was determined using the MTT assay and its possible mechanism of action was investigated through flow cytometry tests. Results: Amlodipine showed MICs ranging from 62.5 to 250 mu g/ml, in addition to action against pre-formed and forming biofilms, with reductions between 50 and 90%. Amlodipine increases the externalization of phosphatidylserine and reduces the cell viability of fungal cells, suggesting apoptosis. Conclusion: Amlodipine had good antifungal activity against planktonic cells and biofilms of Candida spp., by leading the cells to apoptosis.
引用
收藏
页码：1365 / 1375
页数：11
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