Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno-associated virus2/5 Rep protein expression and influences the enrichment fraction of filled capsids

被引：4

作者：

Srinivasan, Prasanna ^{[1
]}

Canova, Christopher T. ^{[2
]}

Sha, Sha ^{[3
]}

Nguyen, Tam N. T. ^{[4
]}

Joseph, John ^{[1
]}

Sangerman, Jose ^{[1
]}

Maloney, Andrew J. ^{[5
]}

Katsikis, Georgios ^{[6
]}

Ou, Rui Wen ^{[7
]}

Hong, Moo Sun ^{[8
]}

Ng, Jaclyn ^{[9
]}

Yuan, Arella ^{[1
]}

Antov, Daniel ^{[1
]}

Song, Sally ^{[1
]}

Chen, Wenyu ^{[1
]}

Neufeld, Caleb ^{[1
]}

Wolfrum, Jacqueline M. ^{[1
]}

Barone, Paul W. ^{[1
]}

Sinskey, Anthony J. ^{[7
]}

Springs, Stacy L. ^{[1
]}

Braatz, Richard D. ^{[1
,2
]}

机构：

[1] MIT, Ctr Biomed Innovat, Cambridge, MA USA

[2] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[3] Ultragenyx Pharmaceut Inc, Cambridge, MA USA

[4] BioNTech SE, Cambridge, MA USA

[5] Amgen Inc, Cambridge, MA USA

[6] Anthol, Cambridge, MA USA

[7] MIT, Dept Biol, Cambridge, MA 02139 USA

[8] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul, South Korea

[9] Stanford Univ, Sch Med, Stanford, CA USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2024年 / 121卷 / 12期

关键词：

Adeno-associated virus; gene therapy; mechanistic modeling; transfection; vector manufacturing; GENE-EXPRESSION; DNA; VECTORS; QUANTIFICATION; REPLICATION; PARTICLES;

D O I：

10.1002/bit.28828

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant adeno-associated virus (rAAV) is a commonly used in vivo gene therapy vector because of its nonpathogenicity, long-term transgene expression, broad tropism, and ability to transduce both dividing and nondividing cells. However, rAAV vector production via transient transfection of mammalian cells typically yields a low fraction of filled-to-total capsids (similar to 1%-30% of total capsids produced). Analysis of our previously developed mechanistic model for rAAV2/5 production attributed these low fill fractions to a poorly coordinated timeline between capsid synthesis and viral DNA replication and the repression of later phase capsid formation by Rep proteins. Here, we extend the model by quantifying the expression dynamics of total Rep proteins and their influence on the key steps of rAAV2/5 production using a multiple dosing transfection of human embryonic kidney 293 (HEK293) cells. We report that the availability of preformed empty capsids and viral DNA copies per cell are not limiting to the capsid-filling reaction. However, optimal expression of Rep proteins (<240 +/- 13 ag per cell) enables enrichment of the filled capsid population (>12% of total capsids/cell) upstream. Our analysis suggests increased enrichment of filled capsids via regulating the expression of Rep proteins is possible but at the expense of per cell capsid titer in a triple plasmid transfection. Our study reveals an intrinsic limitation of scaling rAAV2/5 vector genome (vg) production and underscores the need for approaches that allow for regulating the expression of Rep proteins to maximize vg titer per cell upstream.

引用

页码：3694 / 3714

页数：21

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