Single-cell spatiotemporal analysis of the lungs reveals Slamf9+ macrophages involved in viral clearance and inflammation resolution

被引：7

作者：

Cong, Boyi ^{[1
,2
]}

Dong, Xuan ^{[3
]}

Yang, Zongheng ^{[2
]}

Yu, Pin ^{[4
]}

Chai, Yangyang ^{[2
]}

Liu, Jiaqi ^{[2
]}

Zhang, Meihan ^{[1
]}

Zang, Yupeng ^{[3
]}

Kang, Jingmin ^{[3
]}

Feng, Yu ^{[3
]}

Liu, Yi ^{[3
]}

Feng, Weimin ^{[3
]}

Wang, Dehe ^{[5
]}

Deng, Wei ^{[4
]}

Li, Fengdi ^{[4
]}

Song, Zhiqi ^{[4
]}

Wang, Ziqiao ^{[2
]}

Chen, Xiaosu ^{[1
]}

Qin, Hua ^{[1
]}

Yu, Qinyi ^{[6
]}

Li, Zhiqing ^{[7
,8
]}

Liu, Shuxun ^{[7
,8
]}

Xu, Xun ^{[3
]}

Zhong, Nanshan ^{[8
]}

Ren, Xianwen ^{[5
]}

Qin, Chuan ^{[4
]}

Liu, Longqi ^{[3
]}

Wang, Jian ^{[3
]}

Cao, Xuetao ^{[1
,2
]}

机构：

[1] Nankai Univ, Inst Immunol, Coll Life Sci, State Key Lab Med Chem Biol, Tianjin, Peoples R China

[2] Chinese Acad Med Sci, Peking Union Med Coll, Ctr Immunotherapy, Dept Immunol, Beijing, Peoples R China

[3] BGI Shenzhen, Shenzhen, Guangdong, Peoples R China

[4] Chinese Acad Med Sci, Inst Lab Anim Sci, Beijing, Peoples R China

[5] Changping Lab, Beijing, Peoples R China

[6] Zhejiang Univ, Sch Med, Inst Immunol, Hangzhou, Zhejiang, Peoples R China

[7] Navy Med Univ, Inst Immunol, Natl Key Lab Immun & Inflammat, Shanghai, Peoples R China

[8] Guangzhou Lab, Guangzhou, Guangdong, Peoples R China

来源：

CELL DISCOVERY | 2024年 / 10卷 / 01期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

SARS-COV-2; INFECTION; HAMSTERS; MODEL;

D O I：

10.1038/s41421-024-00734-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

How the lung achieves immune homeostasis after a pulmonary infection is not fully understood. Here, we analyzed the spatiotemporal changes in the lungs over a 2-week natural recovery from severe pneumonia in a Syrian hamster model of SARS-CoV-2 infection. We find that SARS-CoV-2 infects multiple cell types and causes massive cell death at the early stage, including alveolar macrophages. We identify a group of monocyte-derived Slamf9(+) macrophages, which are induced after SARS-CoV-2 infection and resistant to impairment caused by SARS-CoV-2. Slamf9(+) macrophages contain SARS-CoV-2, recruit and interact with Isg12(+)Cst7(+) neutrophils to clear the viruses. After viral clearance, Slamf9(+) macrophages differentiate into Trem2(+) and Fbp1(+) macrophages, contributing to inflammation resolution at the late stage, and finally replenish alveolar macrophages. These findings are validated in a SARS-CoV-2-infected hACE2 mouse model and confirmed with publicly available human autopsy single-cell RNA-seq data, demonstrating the potential role of Slamf9+ macrophages and their coordination with neutrophils in post-injury tissue repair and inflammation resolution.

引用

页数：13