Pycnogenol-Assisted Alleviation of Titanium Dioxide Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation

被引：1

作者：

Lim, Je-Oh ^{[1
,2
,3
]}

Kim, Woong-Il ^{[1
,2
]}

Pak, So-Won ^{[1
,2
]}

Lee, Se-Jin ^{[1
,2
]}

Moon, Changjong ^{[1
,2
]}

Shin, In-Sik ^{[1
,2
]}

Kim, Sung-Hwan ^{[4
]}

Kim, Jong-Choon ^{[1
,2
]}

机构：

[1] Chonnam Natl Univ, Coll Vet Med, Gwangju 61186, South Korea

[2] Chonnam Natl Univ, BK21 FOUR Program, Gwangju 61186, South Korea

[3] Korea Inst Oriental Med, Herbal Med Resources Res Ctr, Naju 58245, South Korea

[4] Korea Inst Toxicol, Jeonbuk Dept Inhalat Res, Jeongup 56212, South Korea

来源：

ANTIOXIDANTS | 2024年 / 13卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

titanium dioxide nanoparticle; lung inflammation; pycnogenol; antioxidative effect; thioredoxin-interacting protein; BARK EXTRACT; AIRWAY INFLAMMATION; OXIDATIVE STRESS; APOPTOSIS; ANTIOXIDANT; CYTOKINES; TOXICITY; EXPOSURE;

D O I：

10.3390/antiox13080972

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Titanium dioxide nanoparticles (TiO2NPs) are used in products that are applied to the human body, such as cosmetics and food, but their biocompatibility remains controversial. Pycnogenol (PYC), a natural extract of pine bark, exerts anti-inflammatory and antioxidant effects. In this study, we investigated whether PYC effectively alleviates pulmonary toxicity induced by airway exposure to TiO2NPs, and the beneficial effects of PYC were explained through the analysis of changes to the mechanism of cytotoxicity. TiO2NPs induced pulmonary inflammation and mucus production, increased the levels of malondialdehyde, and upregulated thioredoxin-interacting protein (TXNIP) and cleaved-caspase 3 (Cas3) in the lungs of mice. However, PYC treatment reduced the levels of all toxicity markers of TiO2NPs and restored glutathione levels. These antioxidant and anti-inflammatory effects of PYC were also demonstrated in TiO2NP-exposed human airway epithelial cells by increasing the mRNA levels of antioxidant enzymes and decreasing the expression of TXNIP, cleaved-Cas3, and inflammatory mediators. Taken together, our results showed that PYC attenuated TiO2NP-induced lung injury via TXNIP downregulation. Therefore, our results suggest the potential of PYC as an effective anti-inflammatory and antioxidant agent against TiO2NP-induced pulmonary toxicity.

引用

页数：15

共 42 条

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