Xenografting Human Musculoskeletal Sarcomas in Mice, Chick Embryo, and Zebrafish: How to Boost Translational Research

被引：1

作者：

Giusti, Veronica ^{[1
]}

Miserocchi, Giacomo ^{[2
]}

Sbanchi, Giulia ^{[1
]}

Pannella, Micaela ^{[1
]}

Hattinger, Claudia Maria ^{[1
]}

Cesari, Marilena ^{[1
]}

Fantoni, Leonardo ^{[1
,3
]}

Guerrieri, Ania Naila ^{[1
]}

Bellotti, Chiara ^{[1
]}

De Vita, Alessandro ^{[2
]}

Spadazzi, Chiara ^{[2
]}

Donati, Davide Maria ^{[4
]}

Torsello, Monica ^{[1
]}

Lucarelli, Enrico ^{[1
]}

Ibrahim, Toni ^{[1
]}

Mercatali, Laura ^{[1
]}

机构：

[1] IRCCS Ist Ortoped Rizzoli, Osteoncol Bone & Soft Tissue Sarcomas & Innovat Th, I-40136 Bologna, Italy

[2] IRCCS Ist Romagnolo Studio Tumori IRST Dino Amador, Biosci Lab, Preclin & Osteoncol Unit, I-47014 Meldola, Italy

[3] Univ Bologna, Dept Med & Surg Sci DIMEC, I-40126 Bologna, Italy

[4] IRCCS Ist Ortoped Rizzoli, Orthopaed Oncol Unit, I-40136 Bologna, Italy

来源：

BIOMEDICINES | 2024年 / 12卷 / 08期

关键词：

patient-derived xenografts (PDX); cell-derived xenografts (CDX); murine models; zebrafish; chorioallantoic membrane (CAM); musculoskeletal sarcomas; PATIENT-DERIVED XENOGRAFTS; FUS-ERG FUSION; HUMAN OSTEOSARCOMA XENOGRAFTS; CHORIOALLANTOIC MEMBRANE CAM; RESISTANT EWINGS-SARCOMA; HUMAN TUMOR XENOGRAFTS; MOUSE MODELS; IN-VITRO; METASTATIC LIPOSARCOMA; ANTITUMOR EFFICACY;

D O I：

10.3390/biomedicines12081921

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Musculoskeletal sarcomas pose major challenges to researchers and clinicians due to their rarity and heterogeneity. Xenografting human cells or tumor fragments in rodents is a mainstay for the generation of cancer models and for the preclinical trial of novel drugs. Lately, though, technical, intrinsic and ethical concerns together with stricter regulations have significantly curbed the employment of murine patient-derived xenografts (mPDX). In alternatives to murine PDXs, researchers have focused on embryonal systems such as chorioallantoic membrane (CAM) and zebrafish embryos. These systems are time- and cost-effective hosts for tumor fragments and near-patient cells. The CAM of the chick embryo represents a unique vascularized environment to host xenografts with high engraftment rates, allowing for ease of visualization and molecular detection of metastatic cells. Thanks to the transparency of the larvae, zebrafish allow for the tracking of tumor development and metastatization, enabling high-throughput drug screening. This review will focus on xenograft models of musculoskeletal sarcomas to highlight the intrinsic and technically distinctive features of the different hosts, and how they can be exploited to elucidate biological mechanisms beneath the different phases of the tumor's natural history and in drug development. Ultimately, the review suggests the combination of different models as an advantageous approach to boost basic and translational research.

引用

页数：30