Fluoride-resistant Streptococcus mutans within cross-kingdom biofilms support Candida albicans growth under fluoride and attenuate the in vitro anti-caries effect of fluorine

被引：1

作者：

Sun, Yan ^{[1
]}

Chen, Yanhan ^{[1
]}

Du, Qian ^{[2
]}

Zhang, Jin ^{[3
,4
]}

Xu, Muxin ^{[1
]}

Zheng, Gaozhe ^{[1
]}

Zhou, Wen ^{[5
]}

Zhou, Xinxuan ^{[3
]}

Qiu, Lili ^{[1
]}

Pan, Yihuai ^{[1
]}

Zhang, Keke ^{[1
]}

机构：

[1] Wenzhou Med Univ, Sch & Hosp Stomatol, Wenzhou, Peoples R China

[2] Zhejiang Univ, Key Lab Oral Biomed Res Zhejiang Prov, Zhejiang Prov Clin Res Ctr Oral Dis, Sch Med,Sch Stomatol,Stomatol Hosp,Canc Ctr, Hangzhou, Peoples R China

[3] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, State Key Lab Oral Dis, Chengdu, Peoples R China

[4] Sichuan Univ, West China Hosp Stomatol, Dept Cariol & Endodont, Chengdu, Peoples R China

[5] Fujian Med Univ, Sch & Hosp Stomatol, Fujian Key Lab Oral Dis, Stomatol Key Lab Fujian Coll & Univ,Fujian Prov En, Fuzhou, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2024年 / 15卷

基金：

中国国家自然科学基金;

关键词：

fluoride-resistant strain; Streptococcus mutans; Candida albicans; cross-kingdom biofilm; caries; fluorine; STRAINS; GENE;

D O I：

10.3389/fmicb.2024.1399525

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fluoride-resistant Streptococcus mutans (S. mutans) might affect the ecological balance of biofilms in the presence of fluoride. We used a S. mutans and Candida albicans (C. albicans) cross-kingdom biofilm model to investigate whether fluoride-resistant S. mutans in biofilms would support C. albicans growth under fluoride stress and attenuate the in vitro anti-caries effect of fluorine. The impact of fluoride-resistant S. mutans on formation of cross-kingdom biofilms by S. mutans and C. albicans in the presence of fluoride was investigated in vitro using the crystal violet staining assay. Biofilm constitution was determined using colony-forming unit (CFU) counts and fluorescent in situ hybridization (FISH). Extracellular polysaccharide (EPS) generation in biofilms was determined by EPS/bacterial dying and water-insoluble polysaccharide detection. Acid production and demineralization were monitored using pH, lactic acid content, and transversal microradiography (TMR). The gene expression of microorganisms in the cross-kingdom biofilm was measured using qRT-PCR. Our results showed that both C. albicans and fluoride-resistant S. mutans grew vigorously, forming robust cross-kingdom biofilms, even in the presence of sodium fluoride (NaF). Moreover, fluoride-resistant S. mutans-containing cross-kingdom biofilms had considerable cariogenic potential for EPS synthesis, acid production, and demineralization ability in the presence of NaF than fluoride-sensitive S. mutans-containing biofilms. Furthermore, the gene expression of microorganisms in the two cross-kingdom biofilms changed dissimilarly in the presence of NaF. In summary, fluoride-resistant S. mutans in cross-kingdom biofilms supported C. albicans growth under fluoride and might attenuate the anti-caries potential of fluorine by maintaining robust cross-kingdom biofilm formation and cariogenic virulence expression in vitro in the presence of NaF.

引用

页数：12

共 1 条

[1] Influence of Fluoride-Resistant Streptococcus mutans Within Antagonistic Dual-Species Biofilms Under Fluoride In Vitro
Zhang, Keke
Xiang, Yangfan
Peng, Youjian
Tang, Fengyu
Cao, Yanfan
Xing, Zhenjie
Li, Yejian
Liao, Xiangyan
Sun, Yan
He, Yan
Ye, Qingsong
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2022, 12

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