Effects of the Allium jesdianum extract on calcium oxalate crystallization with purification and identification of its major compounds

Allium jesdianum, a plant utilized in traditional Iranian medicine, has been historically recognized for its efficacy in countering urolithiasis and alleviating kidney pain. In this study, we investigated the hydroalcoholic extract of A. jesdianum for its potential inhibitory effects on calcium oxalate crystallization (in vitro). The study employed spectrophotometric analysis and microscopic observation to quantify sodium oxalateinduced crystallization, nucleation, and aggregation. The extract of A. jesdianum at concentration of 32 mg/ mL exhibited significant potential in inhibiting crystal growth, aggregation, and reducing crystal particle size. Phytochemical analysis of the extract disclosed the presence of three known phenolic and two identified steroid compounds, offering initial insights into its bioactive constituents. A. jesdianum emerges as a promising candidate for the development of plant-based oxalate anticrystallization drugs. The therapeutic mechanisms might be attributed to pain control and the presence of phenolic compounds acting as free radical scavengers, disrupting stone formation signaling pathways. However, further investigation is required to elucidate specific chemical structures related to these activities. (c) 2024 SAAB. Published by Elsevier B.V. All rights reserved.