Multifarious Two-Photon Fluorescence Microscopy Employing Ultrabroadband Femtosecond Laser Pulses

被引：0

作者：

Isobe, Keisuke ^{[1
]}

Suda, Akira ^{[1
]}

Hashimoto, Hiroshi ^{[2
,3
]}

Kannari, Fumihiko ^{[2
,3
]}

Kawano, Hiroyuki ^{[3
]}

Mizuno, Hideaki

Miyawaki, Atsushi

Midorikawa, Katsumi ^{[1
]}

机构：

[1] RIKEN, Adv Sci Inst, Laser Technol Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan

[2] Keio Univ, Dept Elect & Elect Engn, Yokohama, Kanagawa 2238522, Japan

[3] RIKEN Brain Sci Institute, Lab Cell Funct Dynam, Wako, Saitama 3510198, Japan

来源：

FRONTIERS IN PHYSICS-BOOK | 2009年 / 1150卷

关键词：

Fluorescence; Biomedical imaging; Multiphoton processes; MULTIPHOTON INTRAPULSE INTERFERENCE; EXCITATION; PROTEINS; INDICATORS; CA2+;

D O I：

暂无

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We demonstrate two-photon excited fluorescence (TPEF) microscopy employing the novel spectral phase modulation, which permits not only the selective excitation but also the simultaneous excitation together with controlling TPEF intensities from various fluorophores freely. The selective excitation is applied to the sequential dual-color imaging without emission crosstalk and FRET imaging of a HeLa cell. By the simultaneous excitation, dual-color imaging with various signal ratios including equal signal ratio is successfully achieved.

引用

页码：43 / +

页数：3

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