Protocol for differentiation of monocytes and macrophages from human induced pluripotent stem cells

被引：0
|
作者：
Emmerich, Kevin [1 ]
Calcaterra, Francesca [2 ,3 ]
Tang, Xuming [1 ]
Chen, Guibin [1 ]
Pontarini, Elena [2 ,4 ]
Ciceri, Roberta [2 ,3 ]
Yang, Dan [1 ]
Joseph, Benjamin [1 ]
Della Bella, Silvia [2 ,3 ]
Varea, Isabella [1 ]
Mavilio, Domenico [2 ,3 ]
Boehm, Manfred [1 ]
机构：
[1] NHLBI, NIH, Translat Vasc Med Branch, Bethesda, MD 20814 USA
[2] IRCCS Humanitas Res Hosp, Unit Clin & Expt Immunol, I-20089 Rozzano, Italy
[3] Univ Milan, Dept Med Biotechnol & Translat Med BioMeTra, I-20054 Segrate, Italy
[4] William Harvey Res Inst, Ctr Expt Med & Rheumatol, London, England
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 03期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/j.xpro.2024.103217
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Study of disease-relevant immune cells, namely monocytes and macrophages, is limited based on availability of primary tissue, a limitation that can be remedied using human induced pluripotent stem cell (hiPSC) technology. Here, we present a protocol for differentiation of monocytes and macrophages from hiPSCs. We describe steps for hiPSC maintenance, mesoderm lineage induction, hematopoietic progenitor cells (HPCs) commitment and expansion, and myeloid lineage induction. We then detail procedures for monocyte formation and functional macrophage formation and polarization.
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页数：22
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