Generation of human alveolar epithelial type I cells from pluripotent stem cells

被引：19

作者：

Burgess, Claire L. ^{[1
,2
,3
,4
]}

Huang, Jessie ^{[1
,2
,3
,4
]}

Bawa, Pushpinder S. ^{[1
,2
]}

Alysandratos, Konstantinos-Dionysios ^{[1
,2
,3
,4
]}

Minakin, Kasey ^{[1
,2
,3
,4
]}

Ayers, Lauren J. ^{[1
,2
,3
,4
]}

Morley, Michael P. ^{[5
]}

Babu, Apoorva ^{[5
]}

Villacorta-Martin, Carlos ^{[1
,2
]}

Yampolskaya, Maria ^{[6
]}

Hinds, Anne ^{[3
,4
]}

Thapa, Bibek R. ^{[1
,2
,3
,4
]}

Wang, Feiya ^{[1
,2
]}

Matschulat, Adeline ^{[3
,4
,7
]}

Mehta, Pankaj ^{[6
]}

Morrisey, Edward E. ^{[5
]}

Varelas, Xaralabos ^{[1
,2
,3
,4
,7
]}

Kotton, Darrell N. ^{[1
,2
,3
,4
]}

机构：

[1] Boston Univ, Ctr Regenerat Med, Boston, MA 02118 USA

[2] Boston Med Ctr, Boston, MA 02118 USA

[3] Boston Univ, Chobanian & Avedisian Sch Med, Pulm Ctr, Boston, MA 02118 USA

[4] Boston Univ, Chobanian & Avedisian Sch Med, Dept Med, Boston, MA 02118 USA

[5] Univ Penn, Perelman Sch Med, Penn CHOP Lung Biol Inst, Philadelphia, PA 19104 USA

[6] Boston Univ, Dept Phys, Boston, MA 02215 USA

[7] Boston Univ, Chobanian & Avedisian Sch Med, Dept Biochem & Cell Biol, Boston, MA 02118 USA

来源：

CELL STEM CELL | 2024年 / 31卷 / 05期

关键词：

PULMONARY-FIBROSIS; LUNG DEVELOPMENT; IPS CELLS; DIFFERENTIATION; TRANSDIFFERENTIATION; EXPRESSION; EXPOSURE; REGENERATION; PROGENITOR; REGULATORS;

D O I：

10.1016/j.stem.2024.03.017

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Alveolar epithelial type I cells (AT1s) line the gas exchange barrier of the distal lung and have been historically challenging to isolate or maintain in cell culture. Here, we engineer a human in vitro AT1 model system via directed differentiation of induced pluripotent stem cells (iPSCs). We use primary adult AT1 global transcriptomes to suggest benchmarks and pathways, such as Hippo-LATS-YAP/TAZ signaling, enriched in these cells. Next, we generate iPSC-derived alveolar epithelial type II cells (AT2s) and find that nuclear YAP signaling is sufficient to promote a broad transcriptomic shift from AT2 to AT1 gene programs. The resulting cells express a molecular, morphologic, and functional phenotype reminiscent of human AT1 cells, including the capacity to form a flat epithelial barrier producing characteristic extracellular matrix molecules and secreted ligands. Our results provide an in vitro model of human alveolar epithelial differentiation and a potential source of human AT1s.

引用

页码：657 / 675.e8

页数：28

共 84 条

[61] Progenitor identification and SARS-CoV-2 infection in human distal lung organoids [J].

Salahudeen, Ameen A. ;

Choi, Shannon S. ;

Rustagi, Arjun ;

Zhu, Junjie ;

van Unen, Vincent ;

De la O, Sean M. ;

Flynn, Ryan A. ;

Margalef-Catala, Mar ;

Santos, Antonio J. M. ;

Ju, Jihang ;

Batish, Arpit ;

Usui, Tatsuya ;

Zheng, Grace X. Y. ;

Edwards, Caitlin E. ;

Wagar, Lisa E. ;

Luca, Vincent ;

Anchang, Benedict ;

Nagendran, Monica ;

Nguyen, Khanh ;

Hart, Daniel J. ;

Terry, Jessica M. ;

Belgrader, Phillip ;

Ziraldo, Solongo B. ;

Mikkelsen, Tarjei S. ;

Harbury, Pehr B. ;

Glenn, Jeffrey S. ;

Garcia, K. Christopher ;

Davis, Mark M. ;

Baric, Ralph S. ;

Sabatti, Chiara ;

Amieva, Manuel R. ;

Blish, Catherine A. ;

Desai, Tushar J. ;

Kuo, Calvin J. .

NATURE, 2020, 588 (7839) :670-+

[62] On the Topological Complexity of Human Alveolar Epithelial Type 1 Cells [J].

Schneider, Jan Philipp ;

Wrede, Christoph ;

Hegermann, Jan ;

Weibel, Ewald R. ;

Muehlfeld, Christian ;

Ochs, Matthias .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2019, 199 (09) :1153-1156

[63] Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate [J].

Shiraishi, Kazushige ;

Shah, Parisha P. ;

Morley, Michael P. ;

Loebel, Claudia ;

Santini, Garrett T. ;

Katzen, Jeremy ;

Basil, Maria C. ;

Lin, Susan M. ;

Planer, Joseph D. ;

Cantu, Edward ;

Jones, Dakota L. ;

Nottingham, Ana N. ;

Li, Shanru ;

Crdenas-Diaz, Fabian L. ;

Zhou, Su ;

Burdick, Jason A. ;

Jain, Rajan ;

Morrisey, Edward E. .

CELL, 2023, 186 (07) :1478-+

[64] Excision of Reprogramming Transgenes Improves the Differentiation Potential of iPS Cells Generated with a Single Excisable Vector [J].

Sommer, Cesar A. ;

Sommer, Andreia Gianotti ;

Longmire, Tyler A. ;

Christodoulou, Constantina ;

Thomas, Dolly D. ;

Gostissa, Monica ;

Alt, Fred W. ;

Murphy, George J. ;

Kotton, Darrell N. ;

Mostoslavsky, Gustavo .

STEM CELLS, 2010, 28 (01) :64-74

[65] Alveolar regeneration through a Krt8+transitional stem cell state that persists in human lung fibrosis [J].

Strunz, Maximilian ;

Simon, Lukas M. ;

Ansari, Meshal ;

Kathiriya, Jaymin J. ;

Angelidis, Ilias ;

Mayr, Christoph H. ;

Tsidiridis, George ;

Lange, Marius ;

Mattner, Laura F. ;

Yee, Min ;

Ogar, Paulina ;

Sengupta, Arunima ;

Kukhtevich, Igor ;

Schneider, Robert ;

Zhao, Zhongming ;

Voss, Carola ;

Stoeger, Tobias ;

Neumann, Jens H. L. ;

Hilgendorff, Anne ;

Behr, Juergen ;

O'Reilly, Michael ;

Lehmann, Mareike ;

Burgstaller, Gerald ;

Koenigshoff, Melanie ;

Chapman, Harold A. ;

Theis, Fabian J. ;

Schiller, Herbert B. .

NATURE COMMUNICATIONS, 2020, 11 (01)

[66] Comprehensive Integration of Single-Cell Data [J].

Stuart, Tim ;

Butler, Andrew ;

Hoffman, Paul ;

Hafemeister, Christoph ;

Papalexi, Efthymia ;

Mauck, William M., III ;

Hao, Yuhan ;

Stoeckius, Marlon ;

Smibert, Peter ;

Satija, Rahul .

CELL, 2019, 177 (07) :1888-+

[67] TAZ is required for lung alveolar epithelial cell differentiation after injury [J].

Sun, Tianhe ;

Huang, Zhiyu ;

Zhang, Hua ;

Posner, Clara ;

Jia, Guiquan ;

Ramalingam, Thirumalai R. ;

Xu, Min ;

Brightbill, Hans ;

Egen, Jackson G. ;

Dey, Anwesha ;

Arron, Joseph R. .

JCI INSIGHT, 2019, 4 (14)

[68] A census of the lung: CellCards from LungMAP [J].

Sun, Xin ;

Perl, Anne-Karina ;

Li, Rongbo ;

Bell, Sheila M. ;

Sajti, Eniko ;

Kalinichenko, Vladimir V. ;

Kalin, Tanya, V ;

Misra, Ravi S. ;

Deshmukh, Hitesh ;

Clair, Geremy ;

Kyle, Jennifer ;

Alexander, Laura E. Crotty ;

Masso-Silva, Jorge A. ;

Kitzmiller, Joseph A. ;

Wikenheiser-Brokamp, Kathryn A. ;

Deutsch, Gail ;

Guo, Minzhe ;

Du, Yina ;

Morley, Michael P. ;

Valdez, Michael J. ;

Yu, Haoze, V ;

Jin, Kang ;

Bardes, Eric E. ;

Zepp, Jarod A. ;

Neithamer, Terren ;

Basil, Maria C. ;

Zacharias, William J. ;

Verheyden, Jamie ;

Young, Randee ;

Bandyopadhyay, Gautam ;

Lin, Sara ;

Ansong, Charles ;

Adkins, Joshua ;

Salomonis, Nathan ;

Aronow, Bruce J. ;

Xu, Yan ;

Pryhuber, Gloria ;

Whitsett, Jeff ;

Morrisey, Edward E. .

DEVELOPMENTAL CELL, 2022, 57 (01) :112-+

[69] Heterogeneity in Human Induced Pluripotent Stem Cell-derived Alveolar Epithelial Type II Cells Revealed with ABCA3/SFTPC Reporters [J].

Sun, Yuliang L. ;

Hurley, Killian ;

Villacorta-Martin, Carlos ;

Huang, Jessie ;

Hinds, Anne ;

Gopalan, Krithi ;

Caballero, Ignacio S. ;

Russo, Scott J. ;

Kitzmiller, Joseph A. ;

Whitsett, Jeffrey A. ;

Beers, Michael F. ;

Kotton, Darrell N. .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2021, 65 (04) :442-460

[70] Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq [J].

Tirosh, Itay ;

Izar, Benjamin ;

Prakadan, Sanjay M. ;

Wadsworth, Marc H., II ;

Treacy, Daniel ;

Trombetta, John J. ;

Rotem, Asaf ;

Rodman, Christopher ;

Lian, Christine ;

Murphy, George ;

Fallahi-Sichani, Mohammad ;

Dutton-Regester, Ken ;

Lin, Jia-Ren ;

Cohen, Ofir ;

Shah, Parin ;

Lu, Diana ;

Genshaft, Alex S. ;

Hughes, Travis K. ;

Ziegler, Carly G. K. ;

Kazer, Samuel W. ;

Gaillard, Aleth ;

Kolb, Kellie E. ;

Villani, Alexandra-Chloe ;

Johannessen, Cory M. ;

Andreev, Aleksandr Y. ;

Van Allen, Eliezer M. ;

Bertagnolli, Monica ;

Sorger, Peter K. ;

Sullivan, Ryan J. ;

Flaherty, Keith T. ;

Frederick, Dennie T. ;

Jane-Valbuena, Judit ;

Yoon, Charles H. ;

Rozenblatt-Rosen, Orit ;

Shalek, Alex K. ;

Regev, Aviv ;

Garraway, Levi A. .

SCIENCE, 2016, 352 (6282) :189-196

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