Scanning Switch-off Microscopy for Super-Resolution Fluorescence Imaging

被引：1

作者：

Gao, Zhaoshuai ^{[1
,2
,3
,4
]}

Hou, Shangguo ^{[5
,6
]}

Deng, Suhui ^{[5
,7
]}

Liang, Le ^{[5
,8
]}

Wang, Fei ^{[1
,2
]}

Guo, Linjie ^{[5
]}

Fang, Weina ^{[5
]}

Li, Qian ^{[1
,2
]}

Kang, Bin ^{[3
,4
]}

Chen, Hong-Yuan ^{[3
,4
]}

Fan, Chunhai ^{[1
,2
]}

机构：

[1] Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China

[2] Shanghai Jiao Tong Univ, Natl Ctr Translat Med, Shanghai 200240, Peoples R China

[3] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China

[4] Nanjing Univ, Collaborat Innovat Ctr Chem Life Sci, Nanjing 210023, Peoples R China

[5] Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[6] Inst Syst & Phys Biol, Shenzhen Bay Lab, Shenzhen 518055, Peoples R China

[7] Nanchang Univ, Sch Informat Engn, Nanchang 330031, Peoples R China

[8] Wuhan Univ, Inst Adv Studies, Wuhan 430072, Peoples R China

来源：

NANO LETTERS | 2024年 / 24卷 / 39期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

Photon switch-off effects; Confocal microscope; Exposure dose; Super-resolution microscope; CELLULAR STRUCTURES; ABSORPTION; RESOLUTION; EMISSION;

D O I：

10.1021/acs.nanolett.4c02452

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Super-resolution (SR) microscopy provides a revolutionary optical imaging approach by breaking the diffraction limit of light, while the commonly required special instrumentation with complex optical setup hampers its popularity. Here, we present a scanning switch-off microscopy (SSM) concept that exploits the omnipresent switch-off response of fluorophores to enable super-resolution imaging using a commercial confocal microscope. We validated the SSM model with theoretical calculations and experiments. An imaging resolution of similar to 100 nm was obtained for DNA origami nanostructures and cellular cytoskeletons using fluorescent labels of Alexa 405, Alexa 488, Cy3, and Atto 488. Notably, super-resolution imaging of live cells was realized with SSM, by employing a dronpa fluorescent protein as the fluorescent label. In principle, this SSM method can be applied to any excitation laser scanning-based microscope.

引用

页码：12125 / 12132

页数：8

共 42 条

[1] Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting [J].

Ando, R ;

Mizuno, H ;

Miyawaki, A .

SCIENCE, 2004, 306 (5700) :1370-1373

[2] A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM [J].

Castello, Marco ;

Tortarolo, Giorgio ;

Buttafava, Mauro ;

Deguchi, Takahiro ;

Villa, Federica ;

Koho, Sami ;

Pesce, Luca ;

Oneto, Michele ;

Pelicci, Simone ;

Lanzano, Luca ;

Bianchini, Paolo ;

Sheppard, Colin J. R. ;

Diaspro, Alberto ;

Tosi, Alberto ;

Vicidomini, Giuseppe .

NATURE METHODS, 2019, 16 (02) :175-+

[3] Expansion microscopy [J].

Cho, I. ;

Seo, J. Y. ;

Chang, J. .

JOURNAL OF MICROSCOPY, 2018, 271 (02) :123-128

[4] Laser Particle Stimulated Emission Microscopy [J].

Cho, Sangyeon ;

Humar, Matjaz ;

Martino, Nicola ;

Yun, Seok Hyun .

PHYSICAL REVIEW LETTERS, 2016, 117 (19)

[5] Re-scan confocal microscopy: scanning twice for better resolution [J].

De Luca, Giulia M. R. ;

Breedijk, Ronald M. P. ;

Brandt, Rick A. J. ;

Zeelenberg, Christiaan H. C. ;

de Jong, Babette E. ;

Timmermans, Wendy ;

Azar, Leila Nahidi ;

Hoebe, Ron A. ;

Stallinga, Sjoerd ;

Manders, Erik M. M. .

BIOMEDICAL OPTICS EXPRESS, 2013, 4 (11) :2644-2656

[6] 3D sub-diffraction imaging in a conventional confocal configuration by exploiting super-linear emitters [J].

Denkova, Denitza ;

Ploschner, Martin ;

Das, Minakshi ;

Parker, Lindsay M. ;

Zheng, Xianlin ;

Lu, Yiqing ;

Orth, Antony ;

Packer, Nicolle H. ;

Piper, James A. .

NATURE COMMUNICATIONS, 2019, 10 (1)

[7] Multi-Color, Bleaching-Resistant Super-Resolution Optical Fluctuation Imaging with Oligonucleotide-Based Exchangeable Fluorophores [J].

Glogger, Marius ;

Spahn, Christoph ;

Enderlein, Joerg ;

Heilemann, Mike .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2021, 60 (12) :6310-6313

[8] Single-molecule high-resolution imaging with photobleaching [J].

Gordon, MP ;

Ha, T ;

Selvin, PR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (17) :6462-6465

[9]

Gregor I, 2017, NAT METHODS, V14, P1087, DOI [10.1038/NMETH.4467, 10.1038/nmeth.4467]

[10] Breaking Abbe's diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes [J].

Klar, TA ;

Engel, E ;

Hell, SW .

PHYSICAL REVIEW E, 2001, 64 (06) :9-066613

← 1 2 3 4 5 →