Micro PIXE mapping proves a differential distribution and concentration of trace elements in fungal structures of Rhizophagus intraradices

被引:2
作者
Benavidez, M. E. [1 ]
de la Fourniere, E. M. [2 ]
Colombo, R. P. [1 ]
Silvani, V. A. [1 ]
Debray, M. E. [2 ]
Scotti, A. [3 ,4 ,5 ]
Godeas, A. M. [1 ]
机构
[1] Univ Buenos Aires CONICET, Fac Ciencias Exactas & Nat, Dept Biodivers & Biol Expt, Lab Microbiol Suelo,Inst Biodiversidad & Biol Expt, RA-C1428EGA Buenos Aires, Argentina
[2] CAC, Gerencia Invest & Aplicac, Comis Nacl Energia Atom, Av Gral Paz 1499, RA-B1650KNA San Martin, Buenos Aires, Argentina
[3] Int Ctr Earth Sci, Bioenvironm Lab, Comis Nacl Energia Atom, Urquiza 314, RA-M5600 San Rafael, Argentina
[4] Fac Ciencias Exactas, Padre Contreras 1900, RA-5500 Mendoza, Argentina
[5] Nat Univ Nacionalde Cuyo, Padre Contreras 1900, RA-5500 Mendoza, Argentina
关键词
Extraradical mycelium; Spores; R; intraradices; Trace elements; Micro PIXE; ARBUSCULAR MYCORRHIZAL FUNGI; EXTRARADICAL MYCELIUM; HEAVY-METALS; SPORES; PHYTOREMEDIATION; ACCUMULATION; MECHANISMS; GLOMALIN;
D O I
10.1016/j.funbio.2024.08.008
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Arbuscular mycorrhizal (AM) fungi can sequester different potentially toxic elements, such as trace elements (TEs), within their structures to alleviate the toxicity for its host plant and themselves. To elucidate the role of AM fungi in TEs immobilization in the rhizosphere of host plants, it is important to know the TEs distribution in AM fungal structures. In the present study, we investigated the distribution and concentration of TEs within extraradical spores and mycelium of the AM fungus Rhizophagus intraradices, collected from the rhizosphere of Senecio bonariensis plants grown in a soil polluted with multiple TEs, by using Particle-Induced X-ray Emission with a micro-focused beam (micro PIXE). This technique enabled the simultaneous micrometric mapping of elements in a sample. The calculated values were compared with those in the polluted substrate, measured by the Wavelength Dispersive X-ray Fluorescence technique. The highest concentrations of Fe, P, Ti, Mn, Cr, Cu and Zn were found in AM fungal spores, where they were accumulated, while extraradical mycelium was enriched in Cu. Finally, we demonstrated that AM fungi can simultaneously accumulate high amounts of different TEs in their structures, thus reducing the toxicity of these elements to its host plant.
引用
收藏
页码:2089 / 2093
页数:5
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