pH-responsive bionanocomposite pectin grafted with dimethylaminoethyl methacrylate and acrylic acid copolymer along with silver nanoparticles for breast cancer drug delivery

In this study, we developed a pH-responsive hydrogel membrane composed of pectin, dimethylaminoethyl methacrylate (DMAEMA), and acrylic acid (AA) through a free radical-triggered graft copolymerization method. Freshly formed silver nanoparticles (AgNPs) produced from the reduction reaction were embedded in the produced hydrogel. Pectin-grafted dimethylaminoethyl methacrylate and acrylic acid copolymer [poly (DMAEMA-co-AA)-g-pectin] and its bionanocomposite with AgNPs were confirmed by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). The swelling behavior of poly (DMAEMA-co-AA)-g-pectin in solutions with diverse pH values was assessed. For use as a controlled drug delivery agent, the hydrogel was loaded with 5-fluorouracil (5-FU). The incorporation of AgNPs had a great impact on the entrapment efficiency of 5-FU. At pH 7.4, nearly 48% of the 5-FU was released in vitro from the polymeric nanocomposite within 24 h. Moreover, poly (DMAEMA-co-AA)-g-pectin and poly (DMAEMA-co-AA)-g-pectin/AgNPs presented high cytocompatibility toward normal skin fibroblast cells (BJ1), and the drug delivery system strongly inhibited human Caucasian breast adenocarcinoma cells (MCF-7). These results demonstrated that these poly (DMAEMA-co-AA)-g-pectin/AgNPs are an excellent candidate for use in anticancer drug delivery systems.Highlights Free radical polymerization of nanocomposites incorporated with nanoparticles. Nanoparticles improve the bionanocomposites' drug loading. The developed bionanocomposites exhibit control drug release. The novel bionanocomposites acts as an excellent anticancer drug carrier.