Genome-wide identification and drought stress-induced expression analysis of the NHX gene family in potato

被引:4
作者
Ji, Yihong [1 ]
Liu, Zhen [2 ]
Liu, Chang [3 ]
Shao, Ziying [3 ]
Zhang, Ning [3 ]
Suo, Meiqing [3 ]
Liu, Yuhui [2 ]
Wang, Lei [1 ]
机构
[1] Hebei North Univ, Potato Res Ctr, Zhangjiakou, Peoples R China
[2] Hebei North Univ, Coll Agr & Forestry Sci & Technol, Zhangjiakou, Peoples R China
[3] Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou, Peoples R China
关键词
potato; NHX gene family; bioinformatics analysis; expression profile; drought stress; VACUOLAR NA+/H+ ANTIPORTER; ARABIDOPSIS-THALIANA; SALT TOLERANCE; SODIUM-TRANSPORT; K+ HOMEOSTASIS; EMERGING ROLES; PLANT-GROWTH; OVEREXPRESSION; DATABASE; DUPLICATION;
D O I
10.3389/fgene.2024.1396375
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
NHX proteins are transmembrane antiporters belonging to the cation/proton antiporter gene family, with a conserved Na+ (K+)/H+ exchange (PF00999) protein domain. NHXs play a prominent role in plant growth, development, and defense. However, the role of NHX gene family in potato (Solanum tuberosum L.) is yet to be known. In this study, we conducted a genome-wide analysis of the potato NHX gene family. A total of 25 StNHX family members were identified to be unevenly distributed on 10 chromosomes. The proteins ranged in length from 252 to 1,153 amino acids, with molecular masses ranging from 27516.32 to 127860.87 kD, and isoelectric points (pI) ranging from 4.96 to 9.3. Analyses of gene structures and conserved motifs indicated that StNHX genes in the same phylogenetic cluster are conserved. Phylogenetic analysis divided the StNHX genes into three subfamilies (Classes I, II, and III). Synteny analysis indicated that StNHX gene family Class III of NHX and all Arabidopsis thaliana NHXs shared a close evolutionary relationship. Analysis of cis-acting elements in the upstream 1,500 bp promoter region of potato NHX genes showed that these genes could be regulated by light, stress, and hormones such as abscisic acid and gibberellic acid. Protein-protein interaction network analysis indicated that StNHX proteins may participate in the regulation of potato growth and stress response. Besides, To determine a potential role of these genes in tissue development and drought response, we analyzed the RNA-seq data of different DM potato tissues. The results showed that NHX genes exhibited distinct tissue-specific expression patterns. We further examined the expression patterns of StNHX in different tissues (leaves, roots, shoots, tubers, stolons, and flowers) during the flowering stage in 'Jizhangshu NO.8.' potato. The qRT-PCR results further confirmed the importance of StNHX genes in potato plant growth and development. We further analyzed the RNA-seq data (DM potato) under different abiotic stresses (salt, drought, and heat), and found that the expression of StNHX genes was induced under abiotic stress. qRT-PCR analysis of shoots and roots of 'Jizhangshu NO.8.' potato treated for 0, 6, 12, and 24 h with 15% PEG6000 confirmed that the 25 StNHX genes are involved in the response to drought stress in potato. The results of this study may be useful for selecting appropriate candidate genes for the breeding of new drought-tolerant potato varieties. Furthermore, this study lays a foundation for prospective analysis of StNHX gene functions.
引用
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页数:17
相关论文
共 84 条
[61]  
Walker JM., 2005, The Proteomics Protocols Handbook, P571, DOI 10.1385/1-59259-584-7:531
[62]   A vacuolar Na+/H+ antiporter gene, IbNHX2, enhances salt and drought tolerance in transgenic sweetpotato [J].
Wang, Bing ;
Zhai, Hong ;
He, Shaozhen ;
Zhang, Huan ;
Ren, Zhitong ;
Zhang, Dongdong ;
Liu, Qingchang .
SCIENTIA HORTICULTURAE, 2016, 201 :153-166
[63]   Molecular cloning and characterization of a new Na+/H+ antiporter gene from Brassica napus [J].
Wang, J ;
Zuo, KJ ;
Wu, WS ;
Song, J ;
Sun, XF ;
Lin, J ;
Li, XF ;
Tang, KX .
DNA SEQUENCE, 2003, 14 (05) :351-358
[64]   AtNHX5 and AtNHX6 Control Cellular K+ and pH Homeostasis in Arabidopsis: Three Conserved Acidic Residues Are Essential for K+ Transport [J].
Wang, Liguang ;
Wu, Xuexia ;
Liu, Yafen ;
Qiu, Quan-Sheng .
PLOS ONE, 2015, 10 (12)
[65]   Functional Analysis of the Na+, K+/H+ Antiporter PeNHX3 from the Tree Halophyte Populus euphratica in Yeast by Model-Guided Mutagenesis [J].
Wang, Liguang ;
Feng, Xueying ;
Zhao, Hong ;
Wang, Lidong ;
An, Lizhe ;
Qiu, Quan-Sheng .
PLOS ONE, 2014, 9 (08)
[66]   Expression profiling and integrative analysis of the CESA/CSL superfamily in rice [J].
Wang, Lingqiang ;
Guo, Kai ;
Li, Yu ;
Tu, Yuanyuan ;
Hu, Huizhen ;
Wang, Bingrui ;
Cui, Xiaocan ;
Peng, Liangcai .
BMC PLANT BIOLOGY, 2010, 10
[67]  
Wang Ying Wang Ying, 2019, Journal of Fruit Science, V36, P825
[68]   MCScanX: a toolkit for detection and evolutionary analysis of gene synteny and collinearity [J].
Wang, Yupeng ;
Tang, Haibao ;
DeBarry, Jeremy D. ;
Tan, Xu ;
Li, Jingping ;
Wang, Xiyin ;
Lee, Tae-ho ;
Jin, Huizhe ;
Marler, Barry ;
Guo, Hui ;
Kissinger, Jessica C. ;
Paterson, Andrew H. .
NUCLEIC ACIDS RESEARCH, 2012, 40 (07) :e49
[69]   Genome evolution in polyploids [J].
Wendel, JF .
PLANT MOLECULAR BIOLOGY, 2000, 42 (01) :225-249
[70]   The cotton GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays an important role in salt stress [J].
Wu, CA ;
Yang, GD ;
Meng, QW ;
Zheng, CC .
PLANT AND CELL PHYSIOLOGY, 2004, 45 (05) :600-607