An image processing pipeline for electron cryo-tomography in RELION-5

被引:25
作者
Burt, Alister [1 ,2 ]
Toader, Bogdan [1 ]
Warshamanage, Rangana [3 ,4 ]
von Kugelgen, Andriko [1 ]
Pyle, Euan [5 ,6 ,7 ]
Zivanov, Jasenko [1 ]
Kimanius, Dari [1 ,8 ]
Bharat, Tanmay A. M. [1 ]
Scheres, Sjors H. W. [1 ]
机构
[1] MRC Lab Mol Biol, Cambridge Biomed Campus, Cambridge, England
[2] Genentech Inc, Dept Struct Biol, South San Francisco, CA USA
[3] UKRI Sci & Technol Facil Council, Sci Comp Dept, CCP EM, Harwell Campus, Didcot, England
[4] Univ Pittsburgh, Dept Psychiat, Pittsburgh, PA USA
[5] Birkbeck Coll, Inst Struct & Mol Biol, London, England
[6] Francis Crick Inst, London, England
[7] European Mol Biol Lab, Heidelberg, Germany
[8] CZ Imaging Inst, Redwood City, CA USA
基金
英国医学研究理事会; 欧洲研究理事会; 英国生物技术与生命科学研究理事会;
关键词
electron microscopy; electron tomography; image processing; software; 3-DIMENSIONAL RECONSTRUCTION; MICROSCOPY; RESOLUTION; DYNAMO; TOOL; ART;
D O I
10.1002/2211-5463.13873
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target complexes may be localised in noisy, three-dimensional tomographic reconstructions, averaging images of multiple instances of these molecules can lead to structures with sufficient resolution for de novo atomic modelling. Although many research groups have contributed image processing tools for these tasks, a lack of standardisation and interoperability represents a barrier for newcomers to the field. Here, we present an image processing pipeline for electron tomography data in RELION-5, with functionality ranging from the import of unprocessed movies to the automated building of atomic models in the final maps. Our explicit definition of metadata items that describe the steps of our pipeline has been designed for interoperability with other software tools and provides a framework for further standardisation.
引用
收藏
页码:1788 / 1804
页数:17
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