Comprehensive analysis of RNA-5-methylcytosine modification in breast cancer brain metastasis

被引：0

作者：

Cai, Peiying ^{[1
]}

Li, Jichao ^{[2
]}

An, Meng ^{[3
]}

Li, Min ^{[4
,5
]}

Guo, Jianran ^{[4
,5
]}

Li, Jun ^{[4
,5
]}

Li, Xuan ^{[6
]}

Chen, Shen ^{[7
]}

Zhang, Anqi ^{[1
]}

Li, Peng ^{[3
]}

Liu, Yan ^{[3
]}

Zhang, Wei ^{[7
]}

Fu, Bo ^{[4
,5
]}

机构：

[1] Liaocheng Peoples Hosp, Dept Cent Lab, Liaocheng, Peoples R China

[2] Liaocheng Women & Children Hosp, Dept Clin Lab, Liaocheng, Peoples R China

[3] Liaocheng Peoples Hosp, Dept Clin Lab, Liaocheng, Peoples R China

[4] Liaocheng Peoples Hosp, Dept Precis Biomed Key Lab, Liaocheng, Peoples R China

[5] Shandong Prov Key Med & Hlth Lab Precis Med Aging, Liaocheng, Peoples R China

[6] Liaocheng Peoples Hosp, Dept Mol Pharmacol Key Lab, Liaocheng, Peoples R China

[7] Liaocheng Peoples Hosp, Dept Breast & Thyroid Surg, Liaocheng, Peoples R China

来源：

FUTURE ONCOLOGY | 2024年 / 20卷 / 37期

基金：

中国国家自然科学基金;

关键词：

5-methylcytosine (m5C); breast cancer brain metastasis; methylated RNA immunoprecipitation sequencing (MeRIP-seq); molecular mechanism; MESSENGER-RNA; CIRCULAR RNA; 5-METHYLCYTOSINE PROFILES; POTENTIAL BIOMARKERS; OVARIAN-CANCER; IDENTIFICATION; EXPRESSION; TRANSLATION; METHYLOME; BEHAVIOR;

D O I：

10.1080/14796694.2024.2405459

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Aim: To delineate the RNA-5-methylcytosine (m5C) modification of breast cancer brain metastasis (BCBM). Methods: Methylated RNA immunoprecipitation next-generation sequencing (MeRIP-seq) was performed to obtain RNA-m5C patterns of BCBM.Results: 1048 hypermethylation and 1866 hypomethylation m5C peaks were identified in BCBM compared with those in breast cancer. The most significant m5C hypermethylated genes included ENG, SHANK1, IGFN1, EVL and MMP9, whereas the most significant m5C hypomethylated genes included AREG, SAA2, TP53I11, KRT7 and LCN2. MeRIP-qPCR data were concordant with the corresponding MeRIP-seq results in terms of the observed m5C levels. Conjoint analysis identified 190 hyper-up genes characterized by concurrent m5C hypermethylation and up-regulation, alongside 284 hypo-down genes exhibiting both m5C hypomethylation and down-regulation. Conclusion: This study presents the first comprehensive analysis of RNA-m5C modification in BCBM.

引用

页码：2993 / 3008

页数：16

共 65 条

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