Glycyrrhizic acid alleviates concanavalin A-induced acute liver injury by regulating monocyte-derived macrophages

被引:2
作者
Lu, Juan [1 ]
Gu, Xinyu [2 ]
Xue, Chen [1 ]
Shi, Qingmiao [1 ]
Jia, Junjun [3 ]
Cheng, Jinlin [1 ]
Zeng, Yifan [1 ]
Chu, Qingfei [1 ]
Yuan, Xin [1 ]
Bao, Zhengyi [1 ]
Li, Lanjuan [1 ]
机构
[1] Zhejiang Univ, Ctr Infect Dis, Natl Clin Res Ctr Infect Dis,Affiliated Hosp 1,Sch, Collaborat Innovat Ctr Diag & Treatment Infect Dis, Hangzhou, Zhejiang, Peoples R China
[2] Henan Univ Sci & Technol, Affiliated Hosp 1, Coll Clin Med, Dept Oncol, Luoyang 471003, Henan, Peoples R China
[3] Zhejiang Univ, Affiliated Hosp 1, Dept Surg, Sch Med,Div Hepatobiliary & Pancreat Surg, Hangzhou, Zhejiang, Peoples R China
基金
国家重点研发计划;
关键词
Autoimmune hepatitis; Glycyrrhizic acid; Cytometry by time-of-flight; Single-cell RNA sequencing; Monocyte-derived macrophages; AUTOIMMUNE HEPATITIS; CELLS; INFLAMMATION; INHIBITION; PROGRESS; ACTIVATION; MEMBRANE; NECROSIS; LICORICE; REVEALS;
D O I
10.1016/j.phymed.2024.155586
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Autoimmune hepatitis (AIH) is characterized by persistent liver inflammation induced by aberrant immune responses. Glycyrrhizic acid (GA), a prominent bioactive ingredient of licorice, has shown potential as a safe and effective treatment for AIH. However, the immune regulatory mechanism by which GA exerts its therapeutic effect on AIH remains elusive. In this study, we found that GA intervention significantly alleviated ConA-induced acute liver injury in mice. Cytometry by time-of-flight (CyTOF) analysis revealed that GA increased the abundance of anti-inflammatory F4/80loCD11bhiMHCIIhi MoMF-1 and decreased the abundance of pro-inflammatory F4/80loCD11bhiiNOShi MoMF-3. Multiplex immunofluorescence demonstrated the infiltration of MoMFs in liver tissues. Single-cell RNA sequencing (scRNA-seq) analysis indicated that GA facilitated the immune activation in MoMFs, regulated gene expression of diverse cytokines secreted by MoMFs, and played a role in shaping the immune microenvironment. By integrating the results of CyTOF with scRNA-seq, our study comprehensively elucidates the immune landscape of ConA-induced liver injury following GA intervention, advancing the understanding of GA's mechanism of action. However, it is important to note that some single-cell data in this study remain raw and require further processing and annotation. Our findings suggest that GA alleviates ConA-induced acute liver injury by regulating the function of MoMFs, opening potential avenues for AIH treatment and management, and providing a theoretical basis for the design of novel MoMFs-centered immunotherapies.
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页数:16
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